Isolation and Characterization of Microsatellite Markers for Passiflora contracta
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Microsatellite-Enriched Library Construction and Isolation of Microsatellite Markers
3.2. Genotyping and Data Analysis
4. Conclusions
Acknowledgments
References
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Locus | Repeat motif | Primer sequence (5′–3′) | Dye | Ta (°C) | Size (bp) | Genbank |
---|---|---|---|---|---|---|
PC5E11 | (AC)7(AG)6 | F:CTGGTCTTGGATTGTCCTTTG R:CAAAGTAACTGGTGAGCTTAGGG | FAM | 54 | 158–170 | JX575753 |
PC6E8 | (GT)8 | F:TTGCAAATGATAACAAAACACG R:TATCTCGGATTCCCAAAACC | FAM | 53 | 165–181 | JX575754 |
PC6G11 | (GA)10 | F:ACTGGAAGTCAAACGGTGAG R:GGTGGCTCGAAATTCAAATC | FAM | 52 | 207–229 | JX575755 |
PC7H11 | (CTT)13 | F:TGAAATCCCTGTTGTGTGACTC R:TCCTGAGGGGAGCTGTAGTG | FAM | 53 | 169–175 | JX575759 |
PC6D6 | (CT)9 | F:TTTTTGTGAAGGTAATTTGTCA R:CATGTTGCCTCCATGTTTGA | FAM | 50 | 162–168 | JX575757 |
PC7C12 | (AC)7 | F:TGAAATCCCTGTTGTGTGACTC R:TCCTGAGGGGAGCTGTAGTG | FAM | 55 | 179–195 | JX575758 |
PC6F7 | (CT)8 | F:AACGCATTTTTCAGTTTCTGC R:TGAGACTCCCATTCACCAAG | FAM | 53 | 230–248 | JX575756 |
Passiflora contracta | ||||||
---|---|---|---|---|---|---|
Locus | Linhares-ES | Maraú-BA | ||||
A | HE | HO | A | HE | HO | |
PC5E11# | 6 | 0.82011 | 0.64286 | 5 | 0.63678 | 0.60000 |
PC6E8 | 2 | 0.31452 | 0.37500 | 5 | 0.46508 | 0.55556 |
PC6G11# | 4 | 0.37619 | 0.44444 | 8 | 0.58571 | 0.44444 |
PC7H11 | 3 | 0.60484 | 0.75000 | 3 | 0.65238 | 0.38889* |
PC6D6 | 4 | 0.64368 | 0.53333 | 4 | 0.64308 | 0.53846 |
PC7C12 | 7 | 0.79637 | 0.68750 | 5 | 0.67137 | 0.56250 |
PC6F7 | 9 | 0.84135 | 0.41176* | 4 | 0.57619 | 0.50000 |
mean | 5 | 4.8 |
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Cazé, A.L.R.; Kriedt, R.A.; Beheregaray, L.B.; Bonatto, S.L.; Freitas, L.B. Isolation and Characterization of Microsatellite Markers for Passiflora contracta. Int. J. Mol. Sci. 2012, 13, 11343-11348. https://doi.org/10.3390/ijms130911343
Cazé ALR, Kriedt RA, Beheregaray LB, Bonatto SL, Freitas LB. Isolation and Characterization of Microsatellite Markers for Passiflora contracta. International Journal of Molecular Sciences. 2012; 13(9):11343-11348. https://doi.org/10.3390/ijms130911343
Chicago/Turabian StyleCazé, Ana Luíza R., Raquel A. Kriedt, Luciano B. Beheregaray, Sandro L. Bonatto, and Loreta B. Freitas. 2012. "Isolation and Characterization of Microsatellite Markers for Passiflora contracta" International Journal of Molecular Sciences 13, no. 9: 11343-11348. https://doi.org/10.3390/ijms130911343