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Int. J. Mol. Sci. 2012, 13(9), 11312-11322; doi:10.3390/ijms130911312
Article

Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus)

1,* , 2,3,* , 2
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 and 1
Received: 16 July 2012; in revised form: 26 July 2012 / Accepted: 26 July 2012 / Published: 11 September 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: Fish oil was extracted from the viscera of African Catfish using supercritical carbon dioxide (SC-CO2). A Central Composite Design of Response Surface methodology (RSM) was employed to optimize the SC-CO2 extraction parameters. The oil yield (Y) as response variable was executed against the four independent variables, namely pressure, temperature, flow rate and soaking time. The oil yield varied with the linear, quadratic and interaction of pressure, temperature, flow rate and soaking time. Optimum points were observed within the variables of temperature from 35 °C to 80 °C, pressure from 10 MPa to 40 MPa, flow rate from 1 mL/min to 3 mL/min and soaking time from 1 h to 4 h. However, the extraction parameters were found to be optimized at temperature 57.5 °C, pressure 40 MPa, flow rate 2.0 mL/min and soaking time 2.5 h. At this optimized condition, the highest oil yields were found to be 67.0% (g oil/100 g sample on dry basis) in the viscera of catfish which was reasonable to the yields of 78.0% extracted using the Soxhlet method.
Keywords: supercritical fluid extraction; catfish fish oil; viscera; response surface methodology supercritical fluid extraction; catfish fish oil; viscera; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sarker, M.Z.I.; Selamat, J.; Habib, A.S.M.A.; Ferdosh, S.; Akanda, M.J.H.; Jaffri, J.M. Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus). Int. J. Mol. Sci. 2012, 13, 11312-11322.

AMA Style

Sarker MZI, Selamat J, Habib ASMA, Ferdosh S, Akanda MJH, Jaffri JM. Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus). International Journal of Molecular Sciences. 2012; 13(9):11312-11322.

Chicago/Turabian Style

Sarker, Mohamed Zaidul Islam; Selamat, Jinap; Habib, Abu Sayem Md. Ahsan; Ferdosh, Sahena; Akanda, Mohamed Jahurul Haque; Jaffri, Juliana Mohamed. 2012. "Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus)." Int. J. Mol. Sci. 13, no. 9: 11312-11322.


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