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An iRGD Based Strategy to Study Electrochemically the Species Inside a Cell
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
* Author to whom correspondence should be addressed.
Received: 15 May 2012; in revised form: 27 June 2012 / Accepted: 9 August 2012 / Published: 21 August 2012
Abstract: This paper reports a method for electrical communication between the inner part of cells and an electrode with the help of iRGD peptide. Due to the enhancement of the cell penetration caused by iRGD peptide, DNA molecules, previously modified on a gold electrode surface, can be easily transfected into the cells. At the same time, doxorubicin, an anticancer drug, can also be transfected into cells with high penetration. Consequently, doxorubicin binds to DNA chains through electrostatic interaction, and the redox reaction is transferred out of the cell across the cell membrane. As a result, this work may provide a novel way to get information from inside of cells.
Keywords: iRGD; cell penetration; doxorubicin; cell adhesion; electrochemistry
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Ning, L.; Li, X.; Ding, X.; Yin, Y.; Li, G. An iRGD Based Strategy to Study Electrochemically the Species Inside a Cell. Int. J. Mol. Sci. 2012, 13, 10424-10431.
Ning L, Li X, Ding X, Yin Y, Li G. An iRGD Based Strategy to Study Electrochemically the Species Inside a Cell. International Journal of Molecular Sciences. 2012; 13(8):10424-10431.
Ning, Limin; Li, Xiaoxi; Ding, Xiaorong; Yin, Yongmei; Li, Genxi. 2012. "An iRGD Based Strategy to Study Electrochemically the Species Inside a Cell." Int. J. Mol. Sci. 13, no. 8: 10424-10431.