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• Kuczyńska, A
• Kosmala, A
• Surma, M
• Adamski, T
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• Kuczyńska, A
• Kosmala, A
• Surma, M
• Adamski, T
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• Kuczyńska, A
• Kosmala, A
• Surma, M
• Adamski, T

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Int. J. Mol. Sci. 2012, 13(8), 10410-10423; doi:10.3390/ijms130810410

Article

Identification of Tillering Node Proteins Differentially Accumulated in Barley Recombinant Inbred Lines with Different Juvenile Growth Habits

Anetta Kuczyńska* , Arkadiusz Kosmala, Maria Surma and Tadeusz Adamski

Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska Str. 34, Poznan 60-479, Poland

* Author to whom correspondence should be addressed.

Received: 12 June 2012; in revised form: 8 August 2012 / Accepted: 14 August 2012 / Published: 21 August 2012

(This article belongs to the Special Issue Advances in Proteomic Research)

Download PDF Full-Text [439 KB, uploaded 21 August 2012 09:43 CEST]

Abstract: Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in the living system. Two-dimensional electrophoresis and mass spectrometry were applied to investigate changes in protein abundance associated with different juvenile growth habit as effect of the denso locus in barley homozygous lines derived from a Maresi × Pomo cross combination. A total of 31 protein spots were revealed that demonstrate quantitative differences in protein abundance between the analyzed plants with different juvenile growth habit, and these protein spots were selected to be identified by mass spectrometry. Identification was successful for 27 spots, and functional annotations of proteins revealed that most of them are involved in metabolism and disease/defense-related processes. Functions of the identified proteins and their probable influence on the growth habit in barley are discussed.

Keywords: spring barley; dwarfing gene; denso; 2-D electrophoresis; mass spectrometry; protein abundance

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