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Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice
Department of Histology, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, 1 Feleacului, Arad 310396, Romania
Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania
Food Quality Department, Faculty of Food Processing Technology, Banat’s University of Agricultural Sciences and Veterinary Medicine, 119 Calea Aradului, Timisoara 300645, Romania
* Author to whom correspondence should be addressed.
Received: 18 May 2012; in revised form: 25 June 2012 / Accepted: 5 July 2012 / Published: 19 July 2012
Abstract: The present study investigated the capacity of formulated Berberis vulgaris extract/β-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/β-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies.
Keywords: Berberis vulgaris extract; β-cyclodextrin; hepatoprotection; oxidative stress
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Hermenean, A.; Popescu, C.; Ardelean, A.; Stan, M.; Hadaruga, N.; Mihali, C.-V.; Costache, M.; Dinischiotu, A. Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice. Int. J. Mol. Sci. 2012, 13, 9014-9034.
Hermenean A, Popescu C, Ardelean A, Stan M, Hadaruga N, Mihali C-V, Costache M, Dinischiotu A. Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice. International Journal of Molecular Sciences. 2012; 13(7):9014-9034.
Hermenean, Anca; Popescu, Cristina; Ardelean, Aurel; Stan, Miruna; Hadaruga, Nicoleta; Mihali, Ciprian-Valentin; Costache, Marieta; Dinischiotu, Anca. 2012. "Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice." Int. J. Mol. Sci. 13, no. 7: 9014-9034.