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Int. J. Mol. Sci. 2012, 13(7), 9014-9034; doi:10.3390/ijms13079014
Article

Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice

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1 Department of Histology, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, 1 Feleacului, Arad 310396, Romania 2 Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania 3 Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania 4 Food Quality Department, Faculty of Food Processing Technology, Banat’s University of Agricultural Sciences and Veterinary Medicine, 119 Calea Aradului, Timisoara 300645, Romania
* Author to whom correspondence should be addressed.
Received: 18 May 2012 / Revised: 25 June 2012 / Accepted: 5 July 2012 / Published: 19 July 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The present study investigated the capacity of formulated Berberis vulgaris extract/β-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/β-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies.
Keywords: Berberis vulgaris extract; β-cyclodextrin; hepatoprotection; oxidative stress Berberis vulgaris extract; β-cyclodextrin; hepatoprotection; oxidative stress
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Hermenean, A.; Popescu, C.; Ardelean, A.; Stan, M.; Hadaruga, N.; Mihali, C.-V.; Costache, M.; Dinischiotu, A. Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice. Int. J. Mol. Sci. 2012, 13, 9014-9034.

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