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Int. J. Mol. Sci. 2012, 13(7), 8819-8833; doi:10.3390/ijms13078819
Article

Identification and Characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) Thermotolerance-Related Gene: Bx-HSP90

1
, 1
, 1,2,*  and 1
Received: 18 May 2012; in revised form: 18 June 2012 / Accepted: 6 July 2012 / Published: 16 July 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Temperatures directly influence the distribution and intensity of pine wilt disease caused by the pine wood nematode, Bursaphelenchus xylophilus. To date, however, little is known about the causation and mechanism of this influence. The molecular chaperone HSP90 is a key component that contributes to survival in the abiotic stress response. In this study, we investigated the relationship between the survival of B. xylophilus and the functionality of the HSP90 gene. Bx-HSP90 was cloned from a suppression subtractive hybridization library. In situ mRNA hybridization showed that Bx-HSP90 was constitutively expressed in response to all of the temperatures tested, and RT-PCR indicated that all of the temperatures could induce Bx-HSP90 transcription, with the highest transcript level detected at 30 °C. The suppression of the Bx-HSP90 transcript by RNA interference led to a 25% reduction in the number of nematodes at 30 °C after 44 h. Sharp declines in the survival of the RNAi-treated nematodes were observed after 8 days at 25 °C, 48 h at 30 °C and 24 h at 35 °C. Both heat shock and the knockdown of Bx-HSP90 hindered the growth of the B. xylophilus populations. The results indicate that Bx-HSP90 is essential for the survival of B. xylophilus, confirming the thermoregulatory function of the gene, and delineate the timeframe and temperature range within which the gene function occurs.
Keywords: Bursaphelenchus xylophilus; HSP90; heat shock; pine wilt; suppression subtractive hybridization Bursaphelenchus xylophilus; HSP90; heat shock; pine wilt; suppression subtractive hybridization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, F.; Wang, Z.; Li, D.; Chen, Q. Identification and Characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) Thermotolerance-Related Gene: Bx-HSP90. Int. J. Mol. Sci. 2012, 13, 8819-8833.

AMA Style

Wang F, Wang Z, Li D, Chen Q. Identification and Characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) Thermotolerance-Related Gene: Bx-HSP90. International Journal of Molecular Sciences. 2012; 13(7):8819-8833.

Chicago/Turabian Style

Wang, Feng; Wang, Zhiying; Li, Danlei; Chen, Qiaoli. 2012. "Identification and Characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) Thermotolerance-Related Gene: Bx-HSP90." Int. J. Mol. Sci. 13, no. 7: 8819-8833.



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