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Isolation and Characterization of Cross-Amplification Microsatellite Panels for Species of Procapra (Bovidae; Antilopinae)
Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Beijing 100101, China
Graduate School of the Chinese Academy of Sciences, No. 19 (A) Yuquan Road, Beijing 100049, China
School of Resources and Environmental Engineering, Anhui University, Hefei 230000, China
College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China
* Author to whom correspondence should be addressed.
Received: 30 May 2012; in revised form: 4 July 2012 / Accepted: 9 July 2012 / Published: 16 July 2012
Abstract: The three Procapra species, Tibetan gazelle (P. picticaudata), Mongolian gazelle (P. gutturosa) and Przewalski’s gazelle (P. przewalskii) are endemic to Asia. Several intraspecific genetic issues have been studied with species-specific microsatellite loci in these Asian gazelles. However, cross-species microsatellite panels are absent, which inhibits comparative conservation and evolutionary studies of the Procapra. In this study, we isolated 20 cross-species microsatellite loci for Procapra from both related species and the genomic library of P. przewalskii. Fifty-three samples of the three gazelles were used to characterize the markers. Allele numbers ranged from three to 20, with a mean of 7.93 per locus. Observed heterozygosity (HO) averaged 0.680 and expected heterozygosity (HE) 0.767. The mean polymorphic information content (PIC) was 0.757 for P. picticaudata, 0.803 for P. gutturosa and 0.590 for P. przewalskii. Nine loci were significantly deviated from Hardy-Weinberg (H-W) equilibrium in the three species. Significant linkage disequilibrium was detected in four pairs of loci in P. przewalskii, five pairs in P. gutturosa and 51 pairs in P. picticaudata. Considering the abundance of published loci and their high success rates of cross-amplification, testing and utilization of loci from related species is efficient for wild species of Bovidae. The cross-species microsatellite loci we developed will facilitate further interspecies genetic studies in Procapra.
Keywords: Procapra; genetic markers; microsatellite; cross-amplification; isolation strategy
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Chen, J.; Li, C.; Yang, J.; Luo, Z.; Tang, S.; Li, F.; Li, C.; Liu, B.; Jiang, Z. Isolation and Characterization of Cross-Amplification Microsatellite Panels for Species of Procapra (Bovidae; Antilopinae). Int. J. Mol. Sci. 2012, 13, 8805-8818.
Chen J, Li C, Yang J, Luo Z, Tang S, Li F, Li C, Liu B, Jiang Z. Isolation and Characterization of Cross-Amplification Microsatellite Panels for Species of Procapra (Bovidae; Antilopinae). International Journal of Molecular Sciences. 2012; 13(7):8805-8818.
Chen, Jing; Li, Chunlin; Yang, Ji; Luo, Zhenhua; Tang, Songhua; Li, Feng; Li, Chunwang; Liu, Bingwan; Jiang, Zhigang. 2012. "Isolation and Characterization of Cross-Amplification Microsatellite Panels for Species of Procapra (Bovidae; Antilopinae)." Int. J. Mol. Sci. 13, no. 7: 8805-8818.