Int. J. Mol. Sci. 2012, 13(7), 8679-8695; doi:10.3390/ijms13078679
Article

A Freshwater Streptomyces, Isolated from Tyume River, Produces a Predominantly Extracellular Glycoprotein Bioflocculant

,
,
,
 and *
Received: 15 May 2012; in revised form: 18 June 2012 / Accepted: 21 June 2012 / Published: 13 July 2012
View Full-Text   |   Download PDF [779 KB, uploaded 19 June 2014]
Abstract: We evaluated bioflocculant production by a freshwater actinobacteria whose 16S rDNA nucleotide sequence was deposited in GenBank as Streptomyces sp. Gansen (accession number HQ537129). Optimum culture conditions for bioflocculant production were an initial medium pH of 6.8, incubation temperature of 30 °C, agitation speed of 160 rpm and an inoculum size of 2% (v/v) of cell density 1.5 × 108 cfu/mL. The carbon, nitrogen and cation sources for optimum bioflocculant production were glucose (89% flocculating activity), ammonium sulfate (76% flocculating activity) and MgCl2. Bioflocculant pyrolysis showed three step decomposition indicative of three components while chemical analyses showed 78% carbohydrate and 22% protein (wt/wt). The mass ratio of neutral sugar, amino sugar and uronic acids was 4.6:2.4:3. FTIR spectrometry indicated the presence of carboxyl, hydroxyl and amino groups, typical for heteropolysaccharide. The bioflocculant showed a lattice structure as seen by SEM imaging. Its high flocculation activity suggests its suitability for industrial applicability.
Keywords: bioflocculant; culture conditions; streptomyces; chemical composition; pyrolysis; SEM imaging
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Nwodo, U.U.; Agunbiade, M.O.; Green, E.; Mabinya, L.V.; Okoh, A.I. A Freshwater Streptomyces, Isolated from Tyume River, Produces a Predominantly Extracellular Glycoprotein Bioflocculant. Int. J. Mol. Sci. 2012, 13, 8679-8695.

AMA Style

Nwodo UU, Agunbiade MO, Green E, Mabinya LV, Okoh AI. A Freshwater Streptomyces, Isolated from Tyume River, Produces a Predominantly Extracellular Glycoprotein Bioflocculant. International Journal of Molecular Sciences. 2012; 13(7):8679-8695.

Chicago/Turabian Style

Nwodo, Uchechukwu U.; Agunbiade, Mayowa O.; Green, Ezekiel; Mabinya, Leonard V.; Okoh, Anthony I. 2012. "A Freshwater Streptomyces, Isolated from Tyume River, Produces a Predominantly Extracellular Glycoprotein Bioflocculant." Int. J. Mol. Sci. 13, no. 7: 8679-8695.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert