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Int. J. Mol. Sci. 2012, 13(7), 8259-8272; doi:10.3390/ijms13078259
Article

Genome-Wide Analysis of DNA Methylation and Expression of MicroRNAs in Breast Cancer Cells

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Received: 30 May 2012; in revised form: 19 June 2012 / Accepted: 27 June 2012 / Published: 3 July 2012
(This article belongs to the Special Issue Advances in Molecular Oncology)
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Abstract: DNA methylation of promoters is linked to transcriptional silencing of protein-coding genes, and its alteration plays important roles in cancer formation. For example, hypermethylation of tumor suppressor genes has been seen in some cancers. Alteration of methylation in the promoters of microRNAs (miRNAs) has also been linked to transcriptional changes in cancers; however, no systematic studies of methylation and transcription of miRNAs have been reported. In the present study, to clarify the relation between DNA methylation and transcription of miRNAs, next-generation sequencing and microarrays were used to analyze the methylation and expression of miRNAs, protein-coding genes, other non-coding RNAs (ncRNAs), and pseudogenes in the human breast cancer cell lines MCF7 and the adriamycin (ADR) resistant cell line MCF7/ADR. DNA methylation in the proximal promoter of miRNAs is tightly linked to transcriptional silencing, as it is with protein-coding genes. In protein-coding genes, highly expressed genes have CpG-rich proximal promoters whereas weakly expressed genes do not. This is only rarely observed in other gene categories, including miRNAs. The present study highlights the epigenetic similarities and differences between miRNA and protein-coding genes.
Keywords: DNA methylation; microRNA; cancer DNA methylation; microRNA; cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Morita, S.; Takahashi, R.-U.; Yamashita, R.; Toyoda, A.; Horii, T.; Kimura, M.; Fujiyama, A.; Nakai, K.; Tajima, S.; Matoba, R.; Ochiya, T.; Hatada, I. Genome-Wide Analysis of DNA Methylation and Expression of MicroRNAs in Breast Cancer Cells. Int. J. Mol. Sci. 2012, 13, 8259-8272.

AMA Style

Morita S, Takahashi R-U, Yamashita R, Toyoda A, Horii T, Kimura M, Fujiyama A, Nakai K, Tajima S, Matoba R, Ochiya T, Hatada I. Genome-Wide Analysis of DNA Methylation and Expression of MicroRNAs in Breast Cancer Cells. International Journal of Molecular Sciences. 2012; 13(7):8259-8272.

Chicago/Turabian Style

Morita, Sumiyo; Takahashi, Ryou-u; Yamashita, Riu; Toyoda, Atsushi; Horii, Takuro; Kimura, Mika; Fujiyama, Asao; Nakai, Kenta; Tajima, Shoji; Matoba, Ryo; Ochiya, Takahiro; Hatada, Izuho. 2012. "Genome-Wide Analysis of DNA Methylation and Expression of MicroRNAs in Breast Cancer Cells." Int. J. Mol. Sci. 13, no. 7: 8259-8272.



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