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Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human Pathogens
Microbiology and Virology Unit, “Vita-Salute” San Raffaele University, Milan 20132, Italy
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 16 March 2012; in revised form: 25 June 2012 / Accepted: 27 June 2012 / Published: 4 July 2012
Abstract: In the last two decades, several phage display-selected monoclonal antibodies (mAbs) have been described in the literature and a few of them have managed to reach the clinics. Among these, the anti-respiratory syncytial virus (RSV) Palivizumab, a phage-display optimized mAb, is the only marketed mAb directed against microbial pathogens. Palivizumab is a clear example of the importance of choosing the most appropriate strategy when selecting or optimizing an anti-infectious mAb. From this perspective, the extreme versatility of phage-display technology makes it a useful tool when setting up different strategies for the selection of mAbs directed against human pathogens, especially when their possible clinical use is considered. In this paper, we review the principal phage display strategies used to select anti-infectious mAbs, with particular attention focused on those used against hypervariable pathogens, such as HCV and influenza viruses.
Keywords: phage display; library construction; monoclonal antibodies; biopanning; hypervariable pathogens
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MDPI and ACS Style
Clementi, N.; Mancini, N.; Solforosi, L.; Castelli, M.; Clementi, M.; Burioni, R. Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human Pathogens. Int. J. Mol. Sci. 2012, 13, 8273-8292.
Clementi N, Mancini N, Solforosi L, Castelli M, Clementi M, Burioni R. Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human Pathogens. International Journal of Molecular Sciences. 2012; 13(7):8273-8292.
Clementi, Nicola; Mancini, Nicasio; Solforosi, Laura; Castelli, Matteo; Clementi, Massimo; Burioni, Roberto. 2012. "Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human Pathogens." Int. J. Mol. Sci. 13, no. 7: 8273-8292.