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Biosysthesis of Corn Starch Palmitate by Lipase Novozym 435
Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
* Author to whom correspondence should be addressed.
Received: 23 April 2012; in revised form: 12 May 2012 / Accepted: 23 May 2012 / Published: 12 June 2012
Abstract: Esterification of starch was carried out to expand the usefulness of starch for a myriad of industrial applications. Lipase B from Candida antarctica, immobilized on macroporous acrylic resin (Novozym 435), was used for starch esterification in two reaction systems: micro-solvent system and solvent-free system. The esterification of corn starch with palmitic acid in the solvent-free system and micro-solvent system gave a degree of substitution (DS) of 1.04 and 0.0072 respectively. Esterification of corn starch with palmitic acid was confirmed by UV spectroscopy and IR spectroscopy. The results of emulsifying property analysis showed that the starch palmitate with higher DS contributes to the higher emulsifying property (67.6%) and emulsion stability (79.6%) than the native starch (5.3% and 3.9%). Modified starch obtained by esterification that possesses emulsifying properties and has long chain fatty acids, like palmitic acid, has been widely used in the food, pharmaceutical and biomedical applications industries.
Keywords: corn starch palmitate; micro-solvent system; solvent-free system; lipase esterification; emulsifying property
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MDPI and ACS Style
Xin, J.-Y.; Wang, Y.; Liu, T.; Lin, K.; Chang, L.; Xia, C.-G. Biosysthesis of Corn Starch Palmitate by Lipase Novozym 435. Int. J. Mol. Sci. 2012, 13, 7226-7236.
Xin J-Y, Wang Y, Liu T, Lin K, Chang L, Xia C-G. Biosysthesis of Corn Starch Palmitate by Lipase Novozym 435. International Journal of Molecular Sciences. 2012; 13(6):7226-7236.
Xin, Jia-Ying; Wang, Yan; Liu, Tie; Lin, Kai; Chang, Le; Xia, Chun-Gu. 2012. "Biosysthesis of Corn Starch Palmitate by Lipase Novozym 435." Int. J. Mol. Sci. 13, no. 6: 7226-7236.