Abstract: The antioxidant activities of ethanolic crude extract (ECE) and its four different solvent sub-fractions (namely, petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and the aqueous fraction (AF) from the receptacles of Nelumbo nucifera Gaertn. (Receptaculum Nelumbinis) were investigated using two in vitro antioxidant assays. BF showed the highest total phenolic content (607.6 mg/g gallic acid equivalents), total flavonoid content (862.7 mg/g rutin equivalents) and total proanthocyanidin content (331.0 mg/g catechin equivalents), accompanied with the highest antioxidant activity compared to other fractions through 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays. Five flavonol glycosides, namely hyperoside (1), isoquercitrin (2), quercetin-3-O-β-D-glucuronide (3), isorhamnetin-3-O-β-D-galactoside (4) and syringetin-3-O-β-D-glucoside (5) were isolated from the Receptaculum Nelumbinis. Compounds 2–5 were isolated for the first time from the Receptaculum Nelumbinis. The five isolated ﬂavone glycosides, particularly compounds 1–3, demonstrated signiﬁcant DPPH and ABTS radical scavenging activity, with IC50 values of 8.9 ± 0.2, 5.2 ± 0.2, 7.5 ± 0.1 for DPPH and 114.2 ± 1.7, 112.8 ± 0.8, 172.5 ± 0.7 µg/mL for ABTS, respectively. These results suggest that Receptaculum Nelumbinis has strong antioxidant potential and may be potentially used as a safe and inexpensive bioactive source of natural antioxidants.
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Wu, Y.-B.; Zheng, L.-J.; Wu, J.-G.; Chen, T.-Q.; Yi, J.; Wu, J.-Z. Antioxidant Activities of Extract and Fractions from Receptaculum Nelumbinis and Related Flavonol Glycosides. Int. J. Mol. Sci. 2012, 13, 7163-7173.
Wu Y-B, Zheng L-J, Wu J-G, Chen T-Q, Yi J, Wu J-Z. Antioxidant Activities of Extract and Fractions from Receptaculum Nelumbinis and Related Flavonol Glycosides. International Journal of Molecular Sciences. 2012; 13(6):7163-7173.
Wu, Yan-Bin; Zheng, Li-Jun; Wu, Jian-Guo; Chen, Ti-Qiang; Yi, Jun; Wu, Jin-Zhong. 2012. "Antioxidant Activities of Extract and Fractions from Receptaculum Nelumbinis and Related Flavonol Glycosides." Int. J. Mol. Sci. 13, no. 6: 7163-7173.