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Int. J. Mol. Sci. 2012, 13(5), 6521-6533; doi:10.3390/ijms13056521
Article

Anti-Proliferation and Anti-Invasion Effects of Diosgenin on Gastric Cancer BGC-823 Cells with HIF-1α shRNAs

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1 Department of Obstetrics and Gynecology, Longhua Hospital Shanghai University of Traditional Chinese medicine, Shanghai 200032, China 2 Department of Traditional Chinese medicine, Shanghai Changzheng Hospital, Senond Military Medical University, Shanghai 200003, China 3 Air Force Center of Aviation Medical Evaluation and Training, Dujiangyan 611833, China 4 Cardiovascular Department, Shanghai ChangHai Hospital, Second Military Medical University, Shanghai 200003, China 5 Shanghai University of Traditional Chinese medicine, Shanghai 201203, China
* Authors to whom correspondence should be addressed.
Received: 22 February 2012 / Revised: 2 April 2012 / Accepted: 9 May 2012 / Published: 24 May 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Drug resistance is a major factor for the limited efficacy of chemotherapy in gastric cancer treatment. Hypoxia-inducible factor-1α (HIF-1α), a central transcriptional factor in hypoxia, is suggested to participate in the resistance. Here, we identified a hypoxia-mimic (cobalt chloride) sensitive gastric cell line BGC-823 to explore whether diosgenin, an aglycone of steroidal saponins, can inhibit cancer cell invasion and survival of solid tumor in a hypoxic mimic microenvironment. We have shown that diosgenin is a potent candidate for decreasing the ability of invasion and survival in cobalt chloride treated BGC-823 cells. In addition, when combined with HIF-1α specific short hairpin RNA (shRNA), diosgenin can inhibit BGC-823 cells more effectively. The anti-invasion role of diosgenin may be related to E-cadherin, integrinα5 and integrinβ6. These results suggest that diosgenin may be a useful compound in controlling gastric cancer cells in hypoxia condition, especially when combined with down-regulated HIF-1α.
Keywords: gastric cancer; HIF-1α; hypoxia; shRNA; diosgenin gastric cancer; HIF-1α; hypoxia; shRNA; diosgenin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Mao, Z.-J.; Tang, Q.-J.; Zhang, C.-A.; Qin, Z.-F.; Pang, B.; Wei, P.-K.; Liu, B.; Chou, Y.-N. Anti-Proliferation and Anti-Invasion Effects of Diosgenin on Gastric Cancer BGC-823 Cells with HIF-1α shRNAs. Int. J. Mol. Sci. 2012, 13, 6521-6533.

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