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Int. J. Mol. Sci. 2012, 13(5), 6521-6533; doi:10.3390/ijms13056521

Anti-Proliferation and Anti-Invasion Effects of Diosgenin on Gastric Cancer BGC-823 Cells with HIF-1α shRNAs

2,* , 4,*  and 5
Received: 22 February 2012 / Revised: 2 April 2012 / Accepted: 9 May 2012 / Published: 24 May 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Drug resistance is a major factor for the limited efficacy of chemotherapy in gastric cancer treatment. Hypoxia-inducible factor-1α (HIF-1α), a central transcriptional factor in hypoxia, is suggested to participate in the resistance. Here, we identified a hypoxia-mimic (cobalt chloride) sensitive gastric cell line BGC-823 to explore whether diosgenin, an aglycone of steroidal saponins, can inhibit cancer cell invasion and survival of solid tumor in a hypoxic mimic microenvironment. We have shown that diosgenin is a potent candidate for decreasing the ability of invasion and survival in cobalt chloride treated BGC-823 cells. In addition, when combined with HIF-1α specific short hairpin RNA (shRNA), diosgenin can inhibit BGC-823 cells more effectively. The anti-invasion role of diosgenin may be related to E-cadherin, integrinα5 and integrinβ6. These results suggest that diosgenin may be a useful compound in controlling gastric cancer cells in hypoxia condition, especially when combined with down-regulated HIF-1α.
Keywords: gastric cancer; HIF-1α; hypoxia; shRNA; diosgenin gastric cancer; HIF-1α; hypoxia; shRNA; diosgenin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mao, Z.-J.; Tang, Q.-J.; Zhang, C.-A.; Qin, Z.-F.; Pang, B.; Wei, P.-K.; Liu, B.; Chou, Y.-N. Anti-Proliferation and Anti-Invasion Effects of Diosgenin on Gastric Cancer BGC-823 Cells with HIF-1α shRNAs. Int. J. Mol. Sci. 2012, 13, 6521-6533.

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