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• Mamdouh, W
• Li, Y
• Shawky, S
• Azzazy, H
• Liu, C
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• Mamdouh, W
• Li, Y
• Shawky, S
• Azzazy, H
• Liu, C
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• Li, Y
• Shawky, S
• Azzazy, H
• Liu, C

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Int. J. Mol. Sci. 2012, 13(6), 6534-6547; doi:10.3390/ijms13066534

Article

Influence of “Glow Discharge Plasma” as an External Stimulus on the Self-Assembly, Morphology and Binding Affinity of Gold Nanoparticle-Streptavidin Conjugates

Wael Mamdouh ^1,* , Yingzhi Li ² , Sherif M. Shawky ¹ , Hassan M. E. Azzazy ^1,3  and Chang-Jun Liu ²

¹ Department of Chemistry, School of Sciences and Engineering, The American University in Cairo (AUC), AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt ² Advanced Nanotechnology Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China ³ Yousef Jameel Science & Technology Research Center, the American University in Cairo, AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt

* Author to whom correspondence should be addressed.

Received: 27 March 2012; in revised form: 2 May 2012 / Accepted: 15 May 2012 / Published: 29 May 2012

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Abstract: In this study, we investigate the influence of glow discharge plasma (GDP) on the self-assembly, morphology and binding affinity of streptavidin coated gold nanoparticles (Au-NP-SV) and biotinylated antibody (bAb) adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate. Atomic force microscope (AFM) was used to image the pre- and post-GDP treated samples. The analysis of the AFM images showed a considerable change in the aggregation and morphology of Au-NP-conjugates after treatment with GDP. To our knowledge, this is the first report on using GDP to enhance and speed-up the aggregation (sintering) of adsorbed NP biomolecular conjugates. These results show a promising route that could be generalized for other NPs and their conjugates. It can also be considered as an alternative and cheap aggregation method for controlling the binding affinity of biomolecular species on different surfaces with interesting applications.

Keywords: self-assembly; gold nanoparticles; interface; glow discharge plasma; atomic force microscopy; molecular aggregates; external stimuli

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