Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2012, 13(5), 6352-6369; doi:10.3390/ijms13056352
Article

Revealing the Anti-Tumor Effect of Artificial miRNA p-27-5p on Human Breast Carcinoma Cell Line T-47D

1
, 2
, 3
, 1
, 1
, 1
, 4,5,*  and 1,*
Received: 9 April 2012; in revised form: 9 May 2012 / Accepted: 18 May 2012 / Published: 23 May 2012
(This article belongs to the Special Issue Advances in Molecular Oncology)
View Full-Text   |   Download PDF [785 KB, uploaded 19 June 2014]
Abstract: microRNAs (miRNAs) cause mRNA degradation or translation suppression of their target genes. Previous studies have found direct involvement of miRNAs in cancer initiation and progression. Artificial miRNAs, designed to target single or multiple genes of interest, provide a new therapeutic strategy for cancer. This study investigates the anti-tumor effect of a novel artificial miRNA, miR P-27-5p, on breast cancer. In this study, we reveal that miR P-27-5p downregulates the differential gene expressions associated with the protein modification process and regulation of cell cycle in T-47D cells. Introduction of this novel artificial miRNA, miR P-27-5p, into breast cell lines inhibits cell proliferation and induces the first “gap” phase (G1) cell cycle arrest in cancer cell lines but does not affect normal breast cells. We further show that miR P-27-5p targets the 3′-untranslated mRNA region (3′-UTR) of cyclin-dependent kinase 4 (CDK4) and reduces both the mRNA and protein level of CDK4, which in turn, interferes with phosphorylation of the retinoblastoma protein (RB1). Overall, our data suggest that the effects of miR p-27-5p on cell proliferation and G1 cell cycle arrest are through the downregulation of CDK4 and the suppression of RB1 phosphorylation. This study opens avenues for future therapies targeting breast cancer.
Keywords: miR P-27-5p; exon array; cyclin-dependent kinase 4; cell cycle; breast cancer; retinoblastoma protein miR P-27-5p; exon array; cyclin-dependent kinase 4; cell cycle; breast cancer; retinoblastoma protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Tseng, C.-W.; Huang, H.-C.; Shih, A.-C.; Chang, Y.-Y.; Hsu, C.-C.; Chang, J.-Y.; Li, W.-H.; Juan, H.-F. Revealing the Anti-Tumor Effect of Artificial miRNA p-27-5p on Human Breast Carcinoma Cell Line T-47D. Int. J. Mol. Sci. 2012, 13, 6352-6369.

AMA Style

Tseng C-W, Huang H-C, Shih A-C, Chang Y-Y, Hsu C-C, Chang J-Y, Li W-H, Juan H-F. Revealing the Anti-Tumor Effect of Artificial miRNA p-27-5p on Human Breast Carcinoma Cell Line T-47D. International Journal of Molecular Sciences. 2012; 13(5):6352-6369.

Chicago/Turabian Style

Tseng, Chien-Wei; Huang, Hsuan-Cheng; Shih, Arthur Chun-Chieh; Chang, Ya-Ya; Hsu, Chung-Cheng; Chang, Jen-Yun; Li, Wen-Hsiung; Juan, Hsueh-Fen. 2012. "Revealing the Anti-Tumor Effect of Artificial miRNA p-27-5p on Human Breast Carcinoma Cell Line T-47D." Int. J. Mol. Sci. 13, no. 5: 6352-6369.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert