Int. J. Mol. Sci. 2012, 13(5), 6073-6088; doi:10.3390/ijms13056073
Article

Evaluation of Antioxidant and Free Radical Scavenging Capacities of Polyphenolics from Pods of Caesalpinia pulcherrima

1 Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan 2 Department of Pharmacy, Taipei Medical University Hospital, 250 Wuxing St., Taipei 11031, Taiwan 3 School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan 4 Department of Pharmacy, Taipei City Hospital, No.145, Zhengzhou Rd., Taipei 10341, Taiwan
* Author to whom correspondence should be addressed.
Received: 27 February 2012; in revised form: 9 May 2012 / Accepted: 10 May 2012 / Published: 18 May 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-D-glucoside (3), methyl 6-O-galloyl-β-D-glucoside (4), methyl 3,6-di-O-galloyl-α-D-glucopyranoside (5), gentisic acid 5-O-α-D-(6'-O-galloyl)glucopyranoside (6), guaiacylglycerol 4-O-β-D-(6'-O-galloyl)glucopyranoside (7), 3-methoxy-4-hydroxyphenol 1-O-β-D-(6'-O-galloyl)glucopyranoside (8), (+)-gallocatechin (9), (+)-catechin (10), (+)-gallocatechin 3-O-gallate (11), myricetin 3-rhamnoside (12), and ampelopsin (13). All isolated compounds were tested for their antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and peroxynitrite radicals scavenging assays. Among those compounds, 11, 12, and 2 exhibited the best DPPH-, hydroxyl-, and peroxynitrite radical-scavenging activities, respectively. Compound 7 is a new compound, and possesses better scavenging activities towards DPPH but has equivalent hydroxyl radical scavenging activity when compared to BHT. The paper is the first report on free radical scavenging properties of components of the fresh pods of Caesalpinia pulcherrima. The results obtained from the current study indicate that the free radical scavenging property of fresh pods of Caesalpinia pulcherrima may be one of the mechanisms by which this herbal medicine is effective in several free radical mediated diseases.
Keywords: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical; hydroxyl radical; peroxynitrite; dihydrorhodamine 123 (DHR 123); butylated hydroxytoluene (BHT)

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MDPI and ACS Style

Hsu, F.-L.; Huang, W.-J.; Wu, T.-H.; Lee, M.-H.; Chen, L.-C.; Lu, H.-J.; Hou, W.-C.; Lin, M.-H. Evaluation of Antioxidant and Free Radical Scavenging Capacities of Polyphenolics from Pods of Caesalpinia pulcherrima. Int. J. Mol. Sci. 2012, 13, 6073-6088.

AMA Style

Hsu F-L, Huang W-J, Wu T-H, Lee M-H, Chen L-C, Lu H-J, Hou W-C, Lin M-H. Evaluation of Antioxidant and Free Radical Scavenging Capacities of Polyphenolics from Pods of Caesalpinia pulcherrima. International Journal of Molecular Sciences. 2012; 13(5):6073-6088.

Chicago/Turabian Style

Hsu, Feng-Lin; Huang, Wei-Jan; Wu, Tzu-Hua; Lee, Mei-Hsien; Chen, Lih-Chi; Lu, Hsiao-Jen; Hou, Wen-Chi; Lin, Mei-Hsiang. 2012. "Evaluation of Antioxidant and Free Radical Scavenging Capacities of Polyphenolics from Pods of Caesalpinia pulcherrima." Int. J. Mol. Sci. 13, no. 5: 6073-6088.

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