Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703
Abstract
:1. Introduction
2. Results and Discussion
2.1. Pokemon-Silencing Sensitizes Human Hepatoma to Anoikis and an Apoptosis Inducer
2.2. Pokemon Enhances Resistance of HL7702 Cells to Anoikis
2.3. Pokemon Prevents Anoikis through Suppression of Bim Expression
2.4. Pokemon Suppresses Bim Expression via Direct Binding to the Bim Promoter
2.5. Discussion
3. Experimental Section
3.1. Cell Lines and Culture
3.2. Plasmid Construction
3.3. Pokemon Silencing by siRNA
3.4. Flow Cytometry Analysis
3.5. Real-Time PCR
3.6. Western Blot
3.7. Chromatin Immunoprecipitation
3.8. Dual Luciferase Reporter Assay
3.9. Cell Counting Assay
4. Conclusions
Acknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
References
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Liu, K.; Liu, F.; Zhang, N.; Liu, S.; Jiang, Y. Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703. Int. J. Mol. Sci. 2012, 13, 5818-5831. https://doi.org/10.3390/ijms13055818
Liu K, Liu F, Zhang N, Liu S, Jiang Y. Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703. International Journal of Molecular Sciences. 2012; 13(5):5818-5831. https://doi.org/10.3390/ijms13055818
Chicago/Turabian StyleLiu, Kun, Feng Liu, Nannan Zhang, Shiying Liu, and Yuyang Jiang. 2012. "Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703" International Journal of Molecular Sciences 13, no. 5: 5818-5831. https://doi.org/10.3390/ijms13055818