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Int. J. Mol. Sci. 2012, 13(5), 5818-5831; doi:10.3390/ijms13055818
Article

Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703

1,2
, 2,* , 2
, 2
 and 1,2,3,*
Received: 6 April 2012; in revised form: 23 April 2012 / Accepted: 7 May 2012 / Published: 15 May 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Pokemon is an important proto-oncogene that plays a critical role in cellular oncogenic transformation and tumorigenesis. Anoikis, which is regulated by Bim-mediated apoptosis, is critical to cancer cell invasion and metastasis. We investigated the role of Pokemon in anoikis, and our results show that Pokemon renders liver cells resistant to anoikis via suppression of Bim transcription. We knocked-down Pokemon in human hepatoma cells QGY7703 with small interfering RNAs (siRNA). Knockdown of Pokemon alone did not significantly affect the growth and survival of QGY7703 cells but notably enhanced their sensitivity to apoptotic stress due to the presence of chemical agents or cell detachment, thereby inducing anoikis, as evidenced by flow cytometry and caspase-3 activity assays. In contrast, ectopic expression of Pokemon in HL7702 cells led to resistance to anoikis. Dual-luciferase reporter and ChIP assays illustrated that Pokemon suppressed Bim transcription via direct binding to its promoter. Our results suggest that Pokemon prevents anoikis through the suppression of Bim expression, which facilitates tumor cell invasion and metastasis. This Pokemon-Bim pathway may be an effective target for therapeutic intervention for cancer.
Keywords: Pokemon; anoikis; Bim; hepatoma Pokemon; anoikis; Bim; hepatoma
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, K.; Liu, F.; Zhang, N.; Liu, S.; Jiang, Y. Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703. Int. J. Mol. Sci. 2012, 13, 5818-5831.

AMA Style

Liu K, Liu F, Zhang N, Liu S, Jiang Y. Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703. International Journal of Molecular Sciences. 2012; 13(5):5818-5831.

Chicago/Turabian Style

Liu, Kun; Liu, Feng; Zhang, Nannan; Liu, Shiying; Jiang, Yuyang. 2012. "Pokemon Silencing Leads to Bim-Mediated Anoikis of Human Hepatoma Cell QGY7703." Int. J. Mol. Sci. 13, no. 5: 5818-5831.


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