In Vitro Antioxidant and Anti-Proliferation Activities of Polysaccharides from Various Extracts of Different Mushrooms
Abstract
:1. Introduction
2. Results and Discussion
2.1. Total Carbohydrate, Phenolics and Protein Content of the Crude Polysaccharides
2.2. Antioxidant Activity
2.2.1. ABTS+ Radical-scavenging Activity
2.2.2. OH· Scavenging Assay
2.2.3. Inhibition of Lipid Peroxidation in Rat Liver Homogenate
2.3. Correlations of Polysaccharides with Antioxidant Activity
2.4. Cytotoxicity and Anti-proliferation Assay
3. Experimental Section
3.1. Chemicals
3.2. Mushrooms
3.3. Crude Polysaccharides Extraction
3.4. The Properties of Crude Polysaccharides
3.4.1. Measurement of Total Carbohydrate Contents
3.4.2. Measurement of Protein Contents
3.4.3. Determination of Total Phenolic Contents
3.5. Antioxidant Activity Assay
3.5.1. ABTS+ Radical-scavenging Activity
3.5.2. OH· Scavenging Assay
3.5.3. Inhibition of Lipid Peroxidation in Rat Liver Homogenate
3.6. Cytotoxicity and Anti-proliferation Assay
3.7. Statistical Analysis
4. Conclusions
References
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Mushrooms | ABTS+ (μM TE/g) | OH· (mg VCE/g) | LPO (mg VCE/g) | |
---|---|---|---|---|
Dictyophora indusiata | Acid extract | 24.28 ± 0.54 a | 60.72 ± 0.83 a | 5.61 ± 0.11 a |
Water extract | 16.68 ± 0.41 b | 127.38 ± 1.58 b | 19.46 ± 0.27 b | |
Alkaline extract | 22.35 ± 0.35 c | 46.01 ± 0.81 c | 14.28 ± 0.28 c | |
Hypsizygus marmoreus | Acid extract | 59.17 ± 0.18 d | 86.91 ± 1.02 d | 21.74 ± 0.78 d |
Water extract | 26.93 ± 0.21 e | 109.42 ± 1.55 e | 4.25 ± 0.23 e | |
Alkaline extract | 45.98 ± 1.77 f,l | 46.61 ± 0.94 c | 1.51 ± 0.24 f | |
Lentinus edodes | Acid extract | 47.23 ± 0.81 f | 68.58 ± 1.44 f | 6.10 ± 0.34 a,g,h |
Water extract | 35.38 ± 0.52 g | 84.54 ± 0.27 g | 6.17 ± 0.16 g,h | |
Alkaline extract | 10.97 ± 0.12 h | 46.48 ± 0.51 c | 13.98 ±0.23 c | |
Russula vinosa Lindblad | Acid extract | 65.98 ± 1.74 i | 83.93 ± 0.27 g | 6.62 ± 0.33 g |
Water extract | 9.65 ± 0.11 j | 96.44 ± 0.39 h | 0.70 ± 0.04 i | |
Alkaline extract | 49.88 ± 2.50 k | 50.58 ± 0.58 i | 1.57 ± 0.06 f | |
Hohenbuehelia serotina | Acid extract | 50.01 ± 1.25 k | 76.41 ± 0.45 j | 18.58 ± 0.41 j |
Water extract | 45.33 ± 0.44 l | 110.64 ± 0.64 e | 33.54 ± 0.49 k | |
Alkaline extract | 41.10 ± 1.33 m | 39.82 ± 0.37 k | 9.21 ± 0.12 l | |
Pleurotus eryngii Quel | Acid extract | - | - | - |
Water extract | 25.41 ± 0.35 a | 100.90 ± 1.00 l | 10.57 ± 0.32 m | |
Alkaline extract | 8.88 ± 0.12 j | 26.49 ± 0.40 m | 1.99 ± 0.08 f | |
Hericium erinaceus | Acid extract | 54.32 ± 0.35 n | 53.50 ± 0.61 n | 19.25 ± 0.34 b |
Water extract | 16.05 ± 0.19 b | 101.25 ± 1.48 l | 14.44 ± 0.34 c | |
Alkaline extract | 31.66 ± 0.38 o | 41.26 ± 0.44 o | 5.97 ± 0.09 a,g | |
Auricularia Auricula | Acid extract | 1.70 ± 0.42 p | 5.06 ± 0.12 p | 8.70 ± 0.51 l |
Water extract | 14.16 ± 0.29 q | 59.71 ± 0.70 a | 4.00 ± 0.13 e | |
Alkaline extract | 4.00 ± 0.03 r | 27.93 ± 0.55 q | 2.63 ± 0.15 n |
Crude polysaccharides | ABTS+· | OH· | LPO | |
---|---|---|---|---|
Crude polysaccharides | 1 | 0.401 ** | 0.129 | 0.102 |
ABTS+· | 0.401 ** | 1 | 0.327 ** | 0.346 ** |
OH· | 0.129 | 0.327 ** | 1 | 0.464 ** |
LPO | 0.102 | 0.346 ** | 0.464 ** | 1 |
Mushrooms | HeLa (% inhibition) | HepG2 (% inhibition) | |
---|---|---|---|
Dictyophora indusiata | Acid extract | 35.23 ± 4.00 | 20.83 ± 2.10 |
Water extract | 34.15 ± 9.70 | 16.37 ± 3.30 | |
Alkaline extract | 37.03 ± 3.87 | 22.62 ± 0.66 | |
Hypsizygus marmoreus | Acid extract | −0.15 ± 0.65 | 28.55 ± 4.78 |
Water extract | 7.74 ± 4.49 | 6.90 ± 1.50 | |
Alkaline extract | 33.03 ± 3.80 | 14.39 ± 2.79 | |
Lentinus edodes | Acid extract | 13.18 ± 7.51 | 13.24 ± 4.16 |
Water extract | 11.33 ± 1.70 | 6.02 ± 2.64 | |
Alkaline extract | 35.28 ± 7.95 | 23.68 ± 3.72 | |
Russula vinosa Lindblad | Acid extract | 27.59 ± 1.95 | 12.60 ± 2.96 |
Water extract | 7.13 ± 1.80 | 9.56 ± 2.73 | |
Alkaline extract | 48.92 ± 4.43 | 14.44 ± 0.75 | |
Hohenbuehelia serotina | Acid extract | 12.97 ± 6.42 | 9.93 ± 2.97 |
Water extract | 21.95 ± 2.01 | 9.01 ± 1.86 | |
Alkaline extract | 32.00 ± 5.62 | 12.41 ± 2.81 | |
Pleurotus eryngii Quel | Acid extract | - | - |
Water extract | 11.74 ± 6.10 | 13.66 ± 1.92 | |
Alkaline extract | 11.38 ± 2.01 | 21.24 ± 2.62 | |
Hericium erinaceus | Acid extract | 17.54 ± 2.52 | 9.47 ± 2.97 |
Water extract | 67.85 ± 0.87 | 0.09 ± 0.47 | |
Alkaline extract | 20.05 ± 6.30 | 15.59 ± 3.39 | |
Auricularia Auricula | Acid extract | 15.13 ± 0.45 | 16.14 ± 3.16 |
Water extract | 9.85 ± 2.46 | 19.45 ± 2.42 | |
Alkaline extract | 21.44 ± 1.29 | 18.44 ± 3.81 |
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Li, X.; Wang, Z.; Wang, L.; Walid, E.; Zhang, H. In Vitro Antioxidant and Anti-Proliferation Activities of Polysaccharides from Various Extracts of Different Mushrooms. Int. J. Mol. Sci. 2012, 13, 5801-5817. https://doi.org/10.3390/ijms13055801
Li X, Wang Z, Wang L, Walid E, Zhang H. In Vitro Antioxidant and Anti-Proliferation Activities of Polysaccharides from Various Extracts of Different Mushrooms. International Journal of Molecular Sciences. 2012; 13(5):5801-5817. https://doi.org/10.3390/ijms13055801
Chicago/Turabian StyleLi, Xiaoyu, Zhenyu Wang, Lu Wang, Elfalleh Walid, and Hua Zhang. 2012. "In Vitro Antioxidant and Anti-Proliferation Activities of Polysaccharides from Various Extracts of Different Mushrooms" International Journal of Molecular Sciences 13, no. 5: 5801-5817. https://doi.org/10.3390/ijms13055801