Genetic Diversity of Pinus nigra Arn. Populations in Southern Spain and Northern Morocco Revealed By Inter-Simple Sequence Repeat Profiles †
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Plant Material and Population Selection
3.2. DNA Extraction
3.3. DNA Amplification
3.4. Data Analysis
4. Conclusions
Acknowledgments
- †This article is dedicated to the memory of Dr. Antonio Del Cerro Barja (1952–2010).
References
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Primer Name | Sequence (5′-3′) | Tm (°C) |
---|---|---|
ISCS14 | AGTGAGTGAGTGAGTGAGTGA | 52 |
ISCS17 | DBDBCACCACCACCACCAC | 62 |
ISCS19 | HVHGTGGTGGTGGTGGTG | 62 |
ISCS20 | DHBCGACGACGACGACGA | 62 |
ISCS21 | BDBACAACAACAACAACA | 52 |
ISCS34 | TGTGTGTGTGTGTGTGRC | 52 |
ISCS41 | CTCCTCCTCCTCCTCCTC | 62 |
ISCS69 | CACACACACACACACAA | 52 |
Population | PPL (%) | na | ne | HE | I |
---|---|---|---|---|---|
TrCU | 70.83 | 1.542 | 1.372 | 0.218 | 0.331 |
ArCU | 79.17 | 1.625 | 1.410 | 0.242 | 0.366 |
PaCU | 29.17 | 0.875 | 1.222 | 0.123 | 0.178 |
TaMO | 54.17 | 1.208 | 1.368 | 0.207 | 0.303 |
MA | 62.50 | 1.375 | 1.273 | 0.170 | 0.268 |
PaCA | 29.17 | 0.875 | 1.239 | 0.130 | 0.185 |
NaCA | 45.83 | 1.167 | 1.326 | 0.180 | 0.262 |
YeCA | 37.50 | 1.083 | 1.228 | 0.133 | 0.199 |
Mean | 51.04 | 1.219 | 1.305 | 0.175 | 0.262 |
TrCU | ArCU | PaCU | TaMO | MA | PaCA | NaCA | YeCA | |
---|---|---|---|---|---|---|---|---|
TrCU | - | 0.037 | 0.079 | 0.249 | 0.267 | 0.223 | 0.252 | 0.244 |
ArCU | 0.040 | - | 0.057 | 0.115 | 0.113 | 0.158 | 0.196 | 0.187 |
PaCU | 0.087 | 0.065 | - | 0.168 | 0.171 | 0.176 | 0.230 | 0.268 |
TaMO | 0.258 | 0.124 | 0.181 | - | 0.019 | 0.111 | 0.121 | 0.116 |
MA | 0.274 | 0.120 | 0.183 | 0.031 | - | 0.121 | 0.161 | 0.177 |
PaCA | 0.233 | 0.168 | 0.191 | 0.126 | 0.134 | - | 0.118 | 0.143 |
NaCA | 0.264 | 0.208 | 0.247 | 0.138 | 0.176 | 0.136 | - | 0.046 |
YeCA | 0.254 | 0.198 | 0.283 | 0.131 | 0.191 | 0.160 | 0.064 | - |
Country, Locality | Site | Population Code | Elev. (m) | Longitude | Latitude | n | Mean Annual Temperature (°C) a | Mean Annual Precipitation (mm) a |
---|---|---|---|---|---|---|---|---|
Spain, Cuenca (CU) | Tragacete | TrCU | 1641 | 1°47′22.68″W | 40°20′32.94″N | 50 | 7.54 | 1247 |
Spain, Cuenca (CU) | Arcas | ArCU | 1099 | 2°04′04.38″W | 39°54′27.00″N | 50 | 10.94 | 735 |
Spain, Cuenca (CU) | Palancares | PaCU | 1185 | 1°57′38.97″W | 40°00′28.86″N | 10 | 10.38 | 818 |
Morocco, Talassemtane (MO) | Talassemtane | TaMO | 1710 | 5°08′26.63″W | 35°08′30.33″N | 10 | 9.86 | 1830 |
Spain, Sierra Mágina (MA) | Sierra Mágina | MA | 1820 | 3°26′50.74″W | 37°42′44.96″N | 10 | 11.51 | 1128 |
Spain, Cazorla (CA) | Palancares | PaCA | 1084 | 2°51′59.90″W | 38°06′8.25″N | 10 | 12.64 | 939 |
Spain, Cazorla (CA) | Navaciazo | NaCA | 1400 | 2°51′43.84″W | 37°53′45.75″N | 10 | 10.45 | 1219 |
Spain, Cazorla (CA) | Yelmo | YeCA | 1565 | 2°39′19.51″W | 38°14′56.97″N | 10 | 9.76 | 975 |
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Rubio-Moraga, A.; Candel-Perez, D.; Lucas-Borja, M.E.; Tiscar, P.A.; Viñegla, B.; Linares, J.C.; Gómez-Gómez, L.; Ahrazem, O. Genetic Diversity of Pinus nigra Arn. Populations in Southern Spain and Northern Morocco Revealed By Inter-Simple Sequence Repeat Profiles. Int. J. Mol. Sci. 2012, 13, 5645-5658. https://doi.org/10.3390/ijms13055645
Rubio-Moraga A, Candel-Perez D, Lucas-Borja ME, Tiscar PA, Viñegla B, Linares JC, Gómez-Gómez L, Ahrazem O. Genetic Diversity of Pinus nigra Arn. Populations in Southern Spain and Northern Morocco Revealed By Inter-Simple Sequence Repeat Profiles. International Journal of Molecular Sciences. 2012; 13(5):5645-5658. https://doi.org/10.3390/ijms13055645
Chicago/Turabian StyleRubio-Moraga, Angela, David Candel-Perez, Manuel E. Lucas-Borja, Pedro A. Tiscar, Benjamin Viñegla, Juan C. Linares, Lourdes Gómez-Gómez, and Oussama Ahrazem. 2012. "Genetic Diversity of Pinus nigra Arn. Populations in Southern Spain and Northern Morocco Revealed By Inter-Simple Sequence Repeat Profiles" International Journal of Molecular Sciences 13, no. 5: 5645-5658. https://doi.org/10.3390/ijms13055645