Aβ-40 Y10F Increases βfibrils Formation but Attenuates the Neurotoxicity of Amyloid-β Peptide
Abstract
:1. Introduction
2. Results and Discussion
2.1. Secondary Structural Changes of Aβ (1–40) Y10F
2.2. Aggregation of Wild Type Aβ (1–40) and Aβ-40 Y10F
2.3. Tyrosine Substitution Rendered Aβ-40 Less Toxic via Reduced Generation of Hydrogen Peroxide
3. Experimental Section
3.1. Materials
3.2. Peptide Preparation
3.3. Circular Dichroism Spectroscopy
3.4. Fluorescence Spectroscopy (ThT Assay)
3.5. Transmission Electron Microscopy
3.6. 1-Anilinonaphthaleine-8-Sulphonic Acid (ANS) Binding
3.7. Primary Neuronal Cultures
3.8. Cell Viability Assay
3.9. Hydrogen Peroxide Assay
4. Conclusions
Acknowledgments
References
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Dai, X.; Chang, P.; Liu, W.; Xu, K.; Sun, Y.; Zhu, S.; Jiang, Z. Aβ-40 Y10F Increases βfibrils Formation but Attenuates the Neurotoxicity of Amyloid-β Peptide. Int. J. Mol. Sci. 2012, 13, 5324-5337. https://doi.org/10.3390/ijms13055324
Dai X, Chang P, Liu W, Xu K, Sun Y, Zhu S, Jiang Z. Aβ-40 Y10F Increases βfibrils Formation but Attenuates the Neurotoxicity of Amyloid-β Peptide. International Journal of Molecular Sciences. 2012; 13(5):5324-5337. https://doi.org/10.3390/ijms13055324
Chicago/Turabian StyleDai, Xueling, Ping Chang, Wenjuan Liu, Ke Xu, Yaxuan Sun, Shigong Zhu, and Zhaofeng Jiang. 2012. "Aβ-40 Y10F Increases βfibrils Formation but Attenuates the Neurotoxicity of Amyloid-β Peptide" International Journal of Molecular Sciences 13, no. 5: 5324-5337. https://doi.org/10.3390/ijms13055324