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Int. J. Mol. Sci. 2012, 13(4), 5230-5241; doi:10.3390/ijms13045230
Article

Enhanced Production of a Novel Cyclic Hexapeptide Antibiotic (NW-G01) by Streptomyces alboflavus 313 Using Response Surface Methodology

1,2,* , 3
, 1,4,5
 and 1,4,5,*
Received: 6 January 2012; in revised form: 16 April 2012 / Accepted: 17 April 2012 / Published: 24 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: NW-G01, produced by Streptomyces alboflavus 313, is a novel cyclic hexapeptide antibiotic with many potential applications, including antimicrobial activity and antitumor agents. This study developed a system for optimizing medium components in order to enhance NW-G01 production. In this study, Plackett-Burman design (PBD) was used to find the key ingredients of medium components, and then response surface methodology (RSM) was implemented to determine their optimal concentrations. The results of PBD revealed that the crucial ingredients related to the production of NW-G01 were (NH4)2SO4, peptone and CaCO3. A prediction model has been built in the experiments of central composite design and response surface methodology, and its validation has been further verified. The optimal medium composition was determined (g/L): corn starch 15, glucose 15, peptone 3.80, (NH4)2SO4 0.06, NaCl 1.5, CaCO3 1.30, MgSO4·7H2O 0.015, K2HPO4·3H2O 0.015, MnCl2·4H2O 0.015, FeSO4·7H2O 0.015, and ZnSO4·7H2O 0.015. Compared with NW-G01 production (5.707 mg/L) in non-optimized fermentation medium, the production of NW-G01 (15.564 mg/L) in optimized fermentation medium had a 2.73-fold increase.
Keywords: NW-G01; medium optimization; Streptomyces alboflavus 313; Plackett-Burman design; response surface methodology NW-G01; medium optimization; Streptomyces alboflavus 313; Plackett-Burman design; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guo, Z.; Shen, L.; Ji, Z.; Wu, W. Enhanced Production of a Novel Cyclic Hexapeptide Antibiotic (NW-G01) by Streptomyces alboflavus 313 Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 5230-5241.

AMA Style

Guo Z, Shen L, Ji Z, Wu W. Enhanced Production of a Novel Cyclic Hexapeptide Antibiotic (NW-G01) by Streptomyces alboflavus 313 Using Response Surface Methodology. International Journal of Molecular Sciences. 2012; 13(4):5230-5241.

Chicago/Turabian Style

Guo, Zhengyan; Shen, Ling; Ji, Zhiqin; Wu, Wenjun. 2012. "Enhanced Production of a Novel Cyclic Hexapeptide Antibiotic (NW-G01) by Streptomyces alboflavus 313 Using Response Surface Methodology." Int. J. Mol. Sci. 13, no. 4: 5230-5241.


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