Int. J. Mol. Sci. 2012, 13(4), 4839-4859; doi:10.3390/ijms13044839
Article

Quercetin and Epigallocatechin Gallate Induce in Vitro a Dose-Dependent Stiffening and Hyperpolarizing Effect on the Cell Membrane of Human Mononuclear Blood Cells

1 Biochemistry Department, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6, TraianVuia Street, Bucharest 020956, Romania 2 Toxicology Department, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6, TraianVuia Street, Bucharest 020956, Romania
* Author to whom correspondence should be addressed.
Received: 6 February 2012; in revised form: 26 March 2012 / Accepted: 6 April 2012 / Published: 17 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The bioactivity of polyphenols is closely linked to their ability to interact with biological membranes. The study evaluates the in vitro effect of quercetin and epigallocatechin on the membrane anisotropy and transmembrane potential of peripheral blood mononuclear cells (PBMCs) isolated from 26 type 2 diabetes mellitus patients compared to 25 age matched controls. The in vitro assays were analyzed in correlation with the biochemical and inflammatory profile of the subjects and with insulin resistance parameters (HOMA-IR, plasma resistin) as well. For type 2 diabetes patients, the increase of HOMA-IR and resistin concentration was associated with a significant decrease of the PBMCs membrane anisotropy. The two tested polyphenols induced a dose-dependent hyperpolarizing effect and stiffening of the cell membranes for all tested subjects. Physiological levels of quercetin and epigallocatechin gallate had the tendency to normalize the PBMCs membrane anisotropy of the cells isolated from diabetes patients, bringing it to the level of cells isolated from normoglycemic ones. Epigallocatechin gallate induced higher effects compared to quercetin on the membranes isolated from subjects with higher cardiovascular risk. The decrease of membrane fluidity and the hyperpolarizing effect could explain the cardiovascular protective action of the tested compounds.
Keywords: membrane fluidity; transmembrane potential; chronic hyperglycemia; quercetin; epigallocatechin gallate; human; in vitro

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MDPI and ACS Style

Margina, D.; Ilie, M.; Gradinaru, D. Quercetin and Epigallocatechin Gallate Induce in Vitro a Dose-Dependent Stiffening and Hyperpolarizing Effect on the Cell Membrane of Human Mononuclear Blood Cells. Int. J. Mol. Sci. 2012, 13, 4839-4859.

AMA Style

Margina D, Ilie M, Gradinaru D. Quercetin and Epigallocatechin Gallate Induce in Vitro a Dose-Dependent Stiffening and Hyperpolarizing Effect on the Cell Membrane of Human Mononuclear Blood Cells. International Journal of Molecular Sciences. 2012; 13(4):4839-4859.

Chicago/Turabian Style

Margina, Denisa; Ilie, Mihaela; Gradinaru, Daniela. 2012. "Quercetin and Epigallocatechin Gallate Induce in Vitro a Dose-Dependent Stiffening and Hyperpolarizing Effect on the Cell Membrane of Human Mononuclear Blood Cells." Int. J. Mol. Sci. 13, no. 4: 4839-4859.

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