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Int. J. Mol. Sci. 2012, 13(4), 4124-4140; doi:10.3390/ijms13044124
Article

Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman

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Received: 5 January 2012; in revised form: 11 February 2012 / Accepted: 19 March 2012 / Published: 29 March 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6β-Benzoyloxy-3α-(3,4,5-trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 μg/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95:5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95:5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis.
Keywords: Erythroxylum caatingae; antimicrobial activity; cytotoxic activity; hemolytic activity; apoptosis Erythroxylum caatingae; antimicrobial activity; cytotoxic activity; hemolytic activity; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Aguiar, J.S.; Araújo, R.O.; do Desterro Rodrigues, M.; Sena, K.X.F.R.; Batista, A.M.; Guerra, M.M.P.; Oliveira, S.L.; Tavares, J.F.; Silva, M.S.; Nascimento, S.C.; da Silva, T.G. Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman. Int. J. Mol. Sci. 2012, 13, 4124-4140.

AMA Style

Aguiar JS, Araújo RO, do Desterro Rodrigues M, Sena KXFR, Batista AM, Guerra MMP, Oliveira SL, Tavares JF, Silva MS, Nascimento SC, da Silva TG. Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman. International Journal of Molecular Sciences. 2012; 13(4):4124-4140.

Chicago/Turabian Style

Aguiar, Jaciana S.; Araújo, Rosilma O.; do Desterro Rodrigues, Maria; Sena, Kêsia X. F. R.; Batista, André M.; Guerra, Maria M. P.; Oliveira, Steno L.; Tavares, Josean F.; Silva, Marcelo S.; Nascimento, Silene C.; da Silva, Teresinha Gonçalves. 2012. "Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman." Int. J. Mol. Sci. 13, no. 4: 4124-4140.


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