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Int. J. Mol. Sci. 2012, 13(3), 3478-3491; doi:10.3390/ijms13033478
Article

Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis

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Received: 14 February 2012; in revised form: 5 March 2012 / Accepted: 5 March 2012 / Published: 13 March 2012
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
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Abstract: The CAPRICE (CPC)-like MYB gene family encodes R3-type MYB transcription factors in Arabidopsis. There are six additional CPC-like MYB sequences in the Arabidopsis genome, including TRYPTICHON (TRY), ENHANCER OF TRY AND CPC1 and 2 (ETC1 and ETC2), ENHANCER OF TRY AND CPC3/CPC-LIKE MYB3 (ETC3/CPL3), and TRICHOMELESS1 and 2 (TCL1 and TCL2). We independently identified CPC-LIKE MYB4 (CPL4), which was found to be identical to TCL2. RT-PCR analysis showed that CPL4 is strongly expressed in shoots, including true leaves, but not in roots. Promoter-GUS analyses indicated that CPL4 is specifically expressed in leaf blades. Although CPC expression was repressed in 35S::ETC1, 35S::ETC2 and 35S::CPL3 backgrounds, CPL4 expression was not affected by ETC1, ETC2 or CPL3 over-expression. Notably, several chimeric transcripts may result from inter-genic alternative splicing of CPL4 and ETC2, two tandemly repeated genes on chromosome II. At least two chimeric transcripts named CPL4-α and CPL4-β are expected to encode complete CPC-like MYB proteins.
Keywords: Arabidopsis; epidermal cell differentiation; MYB; transcription factor; trichome Arabidopsis; epidermal cell differentiation; MYB; transcription factor; trichome
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tominaga-Wada, R.; Nukumizu, Y. Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis. Int. J. Mol. Sci. 2012, 13, 3478-3491.

AMA Style

Tominaga-Wada R, Nukumizu Y. Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis. International Journal of Molecular Sciences. 2012; 13(3):3478-3491.

Chicago/Turabian Style

Tominaga-Wada, Rumi; Nukumizu, Yuka. 2012. "Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis." Int. J. Mol. Sci. 13, no. 3: 3478-3491.



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