Int. J. Mol. Sci. 2012, 13(3), 3478-3491; doi:10.3390/ijms13033478
Article

Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis

Interdisciplinary Research Organization, University of Miyazaki, 1-1, Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan
* Author to whom correspondence should be addressed.
Received: 14 February 2012; in revised form: 5 March 2012 / Accepted: 5 March 2012 / Published: 13 March 2012
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
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Abstract: The CAPRICE (CPC)-like MYB gene family encodes R3-type MYB transcription factors in Arabidopsis. There are six additional CPC-like MYB sequences in the Arabidopsis genome, including TRYPTICHON (TRY), ENHANCER OF TRY AND CPC1 and 2 (ETC1 and ETC2), ENHANCER OF TRY AND CPC3/CPC-LIKE MYB3 (ETC3/CPL3), and TRICHOMELESS1 and 2 (TCL1 and TCL2). We independently identified CPC-LIKE MYB4 (CPL4), which was found to be identical to TCL2. RT-PCR analysis showed that CPL4 is strongly expressed in shoots, including true leaves, but not in roots. Promoter-GUS analyses indicated that CPL4 is specifically expressed in leaf blades. Although CPC expression was repressed in 35S::ETC1, 35S::ETC2 and 35S::CPL3 backgrounds, CPL4 expression was not affected by ETC1, ETC2 or CPL3 over-expression. Notably, several chimeric transcripts may result from inter-genic alternative splicing of CPL4 and ETC2, two tandemly repeated genes on chromosome II. At least two chimeric transcripts named CPL4-α and CPL4-β are expected to encode complete CPC-like MYB proteins.
Keywords: Arabidopsis; epidermal cell differentiation; MYB; transcription factor; trichome

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MDPI and ACS Style

Tominaga-Wada, R.; Nukumizu, Y. Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis. Int. J. Mol. Sci. 2012, 13, 3478-3491.

AMA Style

Tominaga-Wada R, Nukumizu Y. Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis. International Journal of Molecular Sciences. 2012; 13(3):3478-3491.

Chicago/Turabian Style

Tominaga-Wada, Rumi; Nukumizu, Yuka. 2012. "Expression Analysis of an R3-Type MYB Transcription Factor CPC-LIKE MYB4 (TRICHOMELESS2) and CPL4-Related Transcripts in Arabidopsis." Int. J. Mol. Sci. 13, no. 3: 3478-3491.

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