Abstract: The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, 1 and 2 months after pseudopurpurin feeding. Compared with rats in the group 0%, the bone mineral density, and the calcium, magnesium, zinc and manganese concentrations in the rats femur in the group 0.5% increased significantly at 1 month and 2 months after pseudopurpurin feeding. Analytical results of micro-computed tomography showed that the group 0.5% displayed an increase in the trabecular volume fraction, trabecular thickness and trabecular number of the distal femur at 1 and 2 months after pseudopurpurin feeding, and the mean thickness, inner perimeter, outer perimeter, and area of the femur diaphysis were significantly increased at 2 months after pseudopurpurin feeding compared with the group 0%. In parallel, the trabecular separation and structure model index of the distal femur were decreased, compared with the group 0% at 1 and 2 months after pseudopurpurin feeding. In the 0.5% and 0% groups, there was no damage to kidney and liver by histopathology analysis. The long-term feeding of pseudopurpurin is safe for rats. The feeding of 0.5% pseudopurpurin which has specific chemical affinities for calcium for bone improvement and level of bone mineral density, enhances the geometry architecture compared with the 0% group.
Keywords: pseudopurpurin; bone mineral density; bone mineral elements; bone geometry architecture
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Wu, C.-C.; Li, X.-B.; Han, T.-S.; Li, P.; Liu, G.-W.; Wang, W.-Z.; Wang, Z. Dietary Pseudopurpurin Effects on Bone Mineral Density and Bone Geometry Architecture in Rats. Int. J. Mol. Sci. 2012, 13, 3431-3443.
Wu C-C, Li X-B, Han T-S, Li P, Liu G-W, Wang W-Z, Wang Z. Dietary Pseudopurpurin Effects on Bone Mineral Density and Bone Geometry Architecture in Rats. International Journal of Molecular Sciences. 2012; 13(3):3431-3443.
Wu, Chen-Chen; Li, Xiao-Bing; Han, Tie-Suo; Li, Peng; Liu, Guo-Wen; Wang, Wei-Zhong; Wang, Zhe. 2012. "Dietary Pseudopurpurin Effects on Bone Mineral Density and Bone Geometry Architecture in Rats." Int. J. Mol. Sci. 13, no. 3: 3431-3443.