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Int. J. Mol. Sci. 2012, 13(3), 3038-3072; doi:10.3390/ijms13033038
Review

Unraveling the Early Events of Amyloid-β Protein (Aβ) Aggregation: Techniques for the Determination of Aβ Aggregate Size

1
,
2
 and
1,*
1 Ralph E. Martin Department of Chemical Engineering, 3202 Bell Engineering Center, University of Arkansas, Fayetteville, AR 72701, USA 2 Department of Chemical Engineering, 2C02 Swearingen Engineering Center, University of South Carolina, Columbia, SC 29208, USA
* Author to whom correspondence should be addressed.
Received: 20 December 2011 / Revised: 9 February 2012 / Accepted: 23 February 2012 / Published: 7 March 2012
(This article belongs to the Special Issue Protein Aggregation)
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Abstract

The aggregation of proteins into insoluble amyloid fibrils coincides with the onset of numerous diseases. An array of techniques is available to study the different stages of the amyloid aggregation process. Recently, emphasis has been placed upon the analysis of oligomeric amyloid species, which have been hypothesized to play a key role in disease progression. This paper reviews techniques utilized to study aggregation of the amyloid-β protein (Aβ) associated with Alzheimer’s disease. In particular, the review focuses on techniques that provide information about the size or quantity of oligomeric Aβ species formed during the early stages of aggregation, including native-PAGE, SDS-PAGE, Western blotting, capillary electrophoresis, mass spectrometry, fluorescence correlation spectroscopy, light scattering, size exclusion chromatography, centrifugation, enzyme-linked immunosorbent assay, and dot blotting.
Keywords: amyloid; capillary electrophoresis; centrifugation; fluorescence correlation spectroscopy; light scattering; mass spectrometry; polyacrylamide gel electrophoresis; oligomer; size exclusion chromatography; Western blotting amyloid; capillary electrophoresis; centrifugation; fluorescence correlation spectroscopy; light scattering; mass spectrometry; polyacrylamide gel electrophoresis; oligomer; size exclusion chromatography; Western blotting
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pryor, N.E.; Moss, M.A.; Hestekin, C.N. Unraveling the Early Events of Amyloid-β Protein (Aβ) Aggregation: Techniques for the Determination of Aβ Aggregate Size. Int. J. Mol. Sci. 2012, 13, 3038-3072.

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