Int. J. Mol. Sci. 2012, 13(3), 3801-3819; doi:10.3390/ijms13033801
Article

The Effect of Osmolytes on Protein Fibrillation

1,†email, 2email, 2email and 1,* email
Received: 9 February 2012; in revised form: 10 March 2012 / Accepted: 13 March 2012 / Published: 21 March 2012
(This article belongs to the Special Issue Protein Aggregation)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Osmolytes are small molecules that are exploited by cells as a protective system against stress conditions. They favour compact protein states which makes them stabilize globular proteins in vitro and promote folding. Conversely, this preference for compact states promotes aggregation of unstructured proteins. Here we combine a brief review of the effect of osmolytes on protein fibrillation with a report of the effect of osmolytes on the unstructured peptide hormone glucagon. Our results show that osmolytes either accelerate the fibrillation kinetics or leave them unaffected, with the exception of the osmolyte taurine. Furthermore, the osmolytes that affected the shape of the fibrillation time profile led to fibrils with different structure as revealed by CD. The structural changes induced by Pro, Ser and choline-O-sulfate could be due to specific osmolytes binding to the peptides, stabilizing an otherwise labile fibrillation intermediate.
Keywords: glucagon; amyloid; taurine; polymorphism; fibrillation mechanism
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MDPI and ACS Style

Macchi, F.; Eisenkolb, M.; Kiefer, H.; Otzen, D.E. The Effect of Osmolytes on Protein Fibrillation. Int. J. Mol. Sci. 2012, 13, 3801-3819.

AMA Style

Macchi F, Eisenkolb M, Kiefer H, Otzen DE. The Effect of Osmolytes on Protein Fibrillation. International Journal of Molecular Sciences. 2012; 13(3):3801-3819.

Chicago/Turabian Style

Macchi, Francesca; Eisenkolb, Maike; Kiefer, Hans; Otzen, Daniel E. 2012. "The Effect of Osmolytes on Protein Fibrillation." Int. J. Mol. Sci. 13, no. 3: 3801-3819.


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