Int. J. Mol. Sci. 2012, 13(3), 2801-2809; doi:10.3390/ijms13032801

An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase

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Received: 31 December 2011; in revised form: 3 February 2012 / Accepted: 27 February 2012 / Published: 2 March 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenomena related to this species. In this work, by making use of a complex formed by Cu2+ and glutathione, which may exhibit excellent voltammetric response, we have proposed a novel potential electrochemical method for the detection of the enzyme. Results show that in the presence of GGT, the formation of Cu2+-glutathione complex on a working electrode will be disrupted, resulting in greatly depressed electrochemical signals. The primary method exhibits some advantages, such as it being fast, cost-efficient, and conveniently operated. It also has the potential to be further developed as an effective method in the quantitative detection of GGT in real samples.
Keywords: gamma glutamyl transpeptidase; glutathione; copper ions; electrochemical
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, G.; Ni, S.; Zhu, S.; Yang, J.; Yin, Y. An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase. Int. J. Mol. Sci. 2012, 13, 2801-2809.

AMA Style

Chen G, Ni S, Zhu S, Yang J, Yin Y. An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase. International Journal of Molecular Sciences. 2012; 13(3):2801-2809.

Chicago/Turabian Style

Chen, Guifang; Ni, Shengfa; Zhu, Sha; Yang, Jinghua; Yin, Yongmei. 2012. "An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase." Int. J. Mol. Sci. 13, no. 3: 2801-2809.

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