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An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase
Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
* Author to whom correspondence should be addressed.
Received: 31 December 2011; in revised form: 3 February 2012 / Accepted: 27 February 2012 / Published: 2 March 2012
Abstract: Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenomena related to this species. In this work, by making use of a complex formed by Cu2+ and glutathione, which may exhibit excellent voltammetric response, we have proposed a novel potential electrochemical method for the detection of the enzyme. Results show that in the presence of GGT, the formation of Cu2+-glutathione complex on a working electrode will be disrupted, resulting in greatly depressed electrochemical signals. The primary method exhibits some advantages, such as it being fast, cost-efficient, and conveniently operated. It also has the potential to be further developed as an effective method in the quantitative detection of GGT in real samples.
Keywords: gamma glutamyl transpeptidase; glutathione; copper ions; electrochemical
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Chen, G.; Ni, S.; Zhu, S.; Yang, J.; Yin, Y. An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase. Int. J. Mol. Sci. 2012, 13, 2801-2809.
Chen G, Ni S, Zhu S, Yang J, Yin Y. An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase. International Journal of Molecular Sciences. 2012; 13(3):2801-2809.
Chen, Guifang; Ni, Shengfa; Zhu, Sha; Yang, Jinghua; Yin, Yongmei. 2012. "An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase." Int. J. Mol. Sci. 13, no. 3: 2801-2809.