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• Pan, W
• Liao, A
• Zhang, J
• Dong, Z
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• Zhang, J
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• Wei, Z
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• Liao, A
• Zhang, J
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• Wei, Z

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Int. J. Mol. Sci. 2012, 13(2), 2354-2367; doi:10.3390/ijms13022354

Article

Supercritical Carbon Dioxide Extraction of the Oak Silkworm (Antheraea pernyi) Pupal Oil: Process Optimization and Composition Determination

Wen-Juan Pan ¹ , Ai-Mei Liao ^1,2 , Jian-Guo Zhang ¹ , Zeng Dong ¹  and Zhao-Jun Wei ^1,*

¹ School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China ² Department of Life Science, Hefei Normal University, Hefei 230061, China

* Author to whom correspondence should be addressed.

Received: 22 November 2011; in revised form: 19 January 2012 / Accepted: 13 February 2012 / Published: 21 February 2012

(This article belongs to the Special Issue Supercritical Fluid Extraction)

Download PDF Full-Text [601 KB, uploaded 21 February 2012 12:04 CET]

Abstract: Supercritical carbon dioxide (SC-CO₂) extraction of oil from oak silkworm pupae was performed in the present research. Response surface methodology (RSM) was applied to optimize the parameters of SC-CO₂ extraction, including extraction pressure, temperature, time and CO₂ flow rate on the yield of oak silkworm pupal oil (OSPO). The optimal extraction condition for oil yield within the experimental range of the variables researched was at 28.03 MPa, 1.83 h, 35.31 °C and 20.26 L/h as flow rate of CO₂. Under this condition, the oil yield was predicted to be 26.18%. The oak silkworm pupal oil contains eight fatty acids, and is rich in unsaturated fatty acids and α-linolenic acid (ALA), accounting for 77.29% and 34.27% in the total oil respectively.

Keywords: supercritical carbon dioxide; oak silkworm pupal oil; response surface methodology

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