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Int. J. Mol. Sci. 2012, 13(2), 2354-2367; doi:10.3390/ijms13022354
Article

Supercritical Carbon Dioxide Extraction of the Oak Silkworm (Antheraea pernyi) Pupal Oil: Process Optimization and Composition Determination

1
, 1,2
, 1
, 1
 and 1,*
1 School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China 2 Department of Life Science, Hefei Normal University, Hefei 230061, China
* Author to whom correspondence should be addressed.
Received: 22 November 2011 / Revised: 19 January 2012 / Accepted: 13 February 2012 / Published: 21 February 2012
(This article belongs to the Special Issue Supercritical Fluid Extraction)
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Abstract

Supercritical carbon dioxide (SC-CO2) extraction of oil from oak silkworm pupae was performed in the present research. Response surface methodology (RSM) was applied to optimize the parameters of SC-CO2 extraction, including extraction pressure, temperature, time and CO2 flow rate on the yield of oak silkworm pupal oil (OSPO). The optimal extraction condition for oil yield within the experimental range of the variables researched was at 28.03 MPa, 1.83 h, 35.31 °C and 20.26 L/h as flow rate of CO2. Under this condition, the oil yield was predicted to be 26.18%. The oak silkworm pupal oil contains eight fatty acids, and is rich in unsaturated fatty acids and α-linolenic acid (ALA), accounting for 77.29% and 34.27% in the total oil respectively.
Keywords: supercritical carbon dioxide; oak silkworm pupal oil; response surface methodology supercritical carbon dioxide; oak silkworm pupal oil; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pan, W.-J.; Liao, A.-M.; Zhang, J.-G.; Dong, Z.; Wei, Z.-J. Supercritical Carbon Dioxide Extraction of the Oak Silkworm (Antheraea pernyi) Pupal Oil: Process Optimization and Composition Determination. Int. J. Mol. Sci. 2012, 13, 2354-2367.

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