Int. J. Mol. Sci. 2012, 13(2), 1582-1597; doi:10.3390/ijms13021582
Article

Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis

1 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang Selangor, Malaysia 2 Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang, Malaysia 3 Institute of Bioscience, University Putra Malaysia, 43400, UPM Serdang, Malaysia
* Author to whom correspondence should be addressed.
Received: 11 October 2011; in revised form: 30 November 2011 / Accepted: 20 December 2011 / Published: 2 February 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA) pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile.
Keywords: Vanda Mimi Palmer; Lactococcus lactis; isoprenoids; sesquiterpene synthase; orchid; fragrance

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MDPI and ACS Style

Song, A.A.L.; Abdullah, J.O.; Abdullah, M.P.; Shafee, N.; Rahim, R.A. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. Int. J. Mol. Sci. 2012, 13, 1582-1597.

AMA Style

Song AAL, Abdullah JO, Abdullah MP, Shafee N, Rahim RA. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. International Journal of Molecular Sciences. 2012; 13(2):1582-1597.

Chicago/Turabian Style

Song, Adelene Ai Lian; Abdullah, Janna O.; Abdullah, Mohd Puad; Shafee, Norazizah; Rahim, Raha A. 2012. "Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis." Int. J. Mol. Sci. 13, no. 2: 1582-1597.

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