Abstract: Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA) pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile.
Keywords: Vanda Mimi Palmer; Lactococcus lactis; isoprenoids; sesquiterpene synthase; orchid; fragrance
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Song, A.A.L.; Abdullah, J.O.; Abdullah, M.P.; Shafee, N.; Rahim, R.A. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. Int. J. Mol. Sci. 2012, 13, 1582-1597.
Song AAL, Abdullah JO, Abdullah MP, Shafee N, Rahim RA. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. International Journal of Molecular Sciences. 2012; 13(2):1582-1597.
Song, Adelene Ai Lian; Abdullah, Janna O.; Abdullah, Mohd Puad; Shafee, Norazizah; Rahim, Raha A. 2012. "Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis." Int. J. Mol. Sci. 13, no. 2: 1582-1597.