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Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang Selangor, Malaysia
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang, Malaysia
Institute of Bioscience, University Putra Malaysia, 43400, UPM Serdang, Malaysia
* Author to whom correspondence should be addressed.
Received: 11 October 2011; in revised form: 30 November 2011 / Accepted: 20 December 2011 / Published: 2 February 2012
Abstract: Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA) pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile.
Keywords: Vanda Mimi Palmer; Lactococcus lactis; isoprenoids; sesquiterpene synthase; orchid; fragrance
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Song, A.A.L.; Abdullah, J.O.; Abdullah, M.P.; Shafee, N.; Rahim, R.A. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. Int. J. Mol. Sci. 2012, 13, 1582-1597.
Song AAL, Abdullah JO, Abdullah MP, Shafee N, Rahim RA. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. International Journal of Molecular Sciences. 2012; 13(2):1582-1597.
Song, Adelene Ai Lian; Abdullah, Janna O.; Abdullah, Mohd Puad; Shafee, Norazizah; Rahim, Raha A. 2012. "Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis." Int. J. Mol. Sci. 13, no. 2: 1582-1597.