Next Article in Journal
Antinociceptive and Anti-Inflammatory Effects of Octacosanol from the Leaves of Sabicea grisea var. grisea in Mice
Previous Article in Journal
Composition, Structure and Functional Properties of Protein Concentrates and Isolates Produced from Walnut (Juglans regia L.)
Int. J. Mol. Sci. 2012, 13(2), 1582-1597; doi:10.3390/ijms13021582
Article

Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis

1
, 2
, 1
, 2
 and 1,3,*
Received: 11 October 2011; in revised form: 30 November 2011 / Accepted: 20 December 2011 / Published: 2 February 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [578 KB, updated 19 June 2014; original version uploaded 19 June 2014]   |   Browse Figures
Abstract: Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA) pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile.
Keywords: Vanda Mimi Palmer; Lactococcus lactis; isoprenoids; sesquiterpene synthase; orchid; fragrance Vanda Mimi Palmer; Lactococcus lactis; isoprenoids; sesquiterpene synthase; orchid; fragrance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Song, A.A.L.; Abdullah, J.O.; Abdullah, M.P.; Shafee, N.; Rahim, R.A. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. Int. J. Mol. Sci. 2012, 13, 1582-1597.

AMA Style

Song AAL, Abdullah JO, Abdullah MP, Shafee N, Rahim RA. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis. International Journal of Molecular Sciences. 2012; 13(2):1582-1597.

Chicago/Turabian Style

Song, Adelene Ai Lian; Abdullah, Janna O.; Abdullah, Mohd Puad; Shafee, Norazizah; Rahim, Raha A. 2012. "Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis." Int. J. Mol. Sci. 13, no. 2: 1582-1597.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert