Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2012, 13(2), 1598-1611; doi:10.3390/ijms13021598
Article

Antinociceptive and Anti-Inflammatory Effects of Octacosanol from the Leaves of Sabicea grisea var. grisea in Mice

1
, 1
, 2
, 2
, 3
 and 2,*
Received: 21 December 2011; in revised form: 26 January 2012 / Accepted: 30 January 2012 / Published: 2 February 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [268 KB, updated 19 June 2014; original version uploaded 19 June 2014]
Abstract: Sabicea species are used in the Amazon for treatment of fever and malaria, which suggests that its chemical constituents may have some effect on pain and inflammation. Phytochemical analysis of the hexane fraction obtained from the crude ethanol extract from Sabicea grisea var. grisea Cham. & Schltdl (Rubiaceae), an endemic plant in Brazil, resulted in the isolation of octacosanol. This study investigated the antinociceptive and anti-inflammatory effects of the octacosanol in different experimental models. The crude ethanolic extract and hexane fraction obtained from the leaves of S. grisea produced an inhibition of acetic acid-induced pain. Moreover, octacosanol isolated from the hexane fraction produced a significant inhibition of pain response elicited by acetic acid. Pre-treatment with yohimbine, an alpha 2-adrenergic receptor antagonist, notably reversed the antinociceptive activity induced by octacosanol in the abdominal constriction test. Furthermore, mice treated with octacosanol did not exhibit any behavioral alteration during the hot plate and rota-rod tests, indicating non-participation of the supraspinal components in the modulation of pain by octacosanol with no motor abnormality. In the formalin test, octacosanol did not inhibit the licking time in first phase (neurogenic pain), but significantly inhibited the licking time in second phase (inflammatory pain) of mice. The anti-inflammatory effect of octacosanol was evaluated using carrageenan-induced pleurisy. The octacosanol significantly reduced the total leukocyte count and neutrophils influx, as well as TNF-α levels in the carrageenan-induced pleurisy. This study revealed that the mechanism responsible for the antinociceptive and anti-inflammatory effects of the octacosanol appears to be partly associated with an inhibition of alpha 2-adrenergic transmission and an inhibition of pathways dependent on pro-inflammatory cytokines. Finally, these results demonstrated that the octacosanol from the leaves of S. grisea possesses antinociceptive and anti-inflammatory activities, which could be of relevance for the pharmacological control of pain and inflammatory processes.
Keywords: octacosanol; anti-inflammatory; antinociception; TNF-α; Sabicea grisea octacosanol; anti-inflammatory; antinociception; TNF-α; Sabicea grisea
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Oliveira, A.M.; Conserva, L.M.; de Souza Ferro, J.N.; Brito, F.A.; Lemos, R.P.L.; Barreto, E. Antinociceptive and Anti-Inflammatory Effects of Octacosanol from the Leaves of Sabicea grisea var. grisea in Mice. Int. J. Mol. Sci. 2012, 13, 1598-1611.

AMA Style

Oliveira AM, Conserva LM, de Souza Ferro JN, Brito FA, Lemos RPL, Barreto E. Antinociceptive and Anti-Inflammatory Effects of Octacosanol from the Leaves of Sabicea grisea var. grisea in Mice. International Journal of Molecular Sciences. 2012; 13(2):1598-1611.

Chicago/Turabian Style

Oliveira, Anderson Marques de; Conserva, Lucia M.; de Souza Ferro, Jamylle N.; Brito, Fabíola de Almeida; Lemos, Rosângela P. Lyra; Barreto, Emiliano. 2012. "Antinociceptive and Anti-Inflammatory Effects of Octacosanol from the Leaves of Sabicea grisea var. grisea in Mice." Int. J. Mol. Sci. 13, no. 2: 1598-1611.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert