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Int. J. Mol. Sci. 2012, 13(2), 1437-1443; doi:10.3390/ijms13021437

Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China

1
Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China
2
Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan, China
3
Pharmacy and Inspection Department, Sichuan Traditional Chinese Medicine College, Mianyang 621000, Sichuan, China
4
The Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China
*
Authors to whom correspondence should be addressed.
Received: 31 December 2011 / Revised: 16 January 2012 / Accepted: 17 January 2012 / Published: 31 January 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild individuals from Gaoligong Mountains in western Yunnan Province, China. The number of alleles per locus ranged from 2 to 4 with a mean of 2.9. The expected and observed levels of heterozygosity ranged from 0.042 to 0.726 and from 0.000 to 1.000, with averages of 0.44 and 0.31, respectively. These polymorphic microsatellite markers should prove useful in population genetics studies and assessments of genetic variation to develop conservation and management strategies for this species. View Full-Text
Keywords: Cardiocrinum giganteum var. yunnanense; microsatellite markers; polymorphism; population genetics; Liliaceae Cardiocrinum giganteum var. yunnanense; microsatellite markers; polymorphism; population genetics; Liliaceae
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Li, R.; Yang, J.; Yang, J.; Dao, Z. Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China. Int. J. Mol. Sci. 2012, 13, 1437-1443.

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