Int. J. Mol. Sci. 2012, 13(2), 1437-1443; doi:10.3390/ijms13021437
Article

Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China

Rong Li 1 email, Jie Yang 2,3 email, Junbo Yang 4,* email and Zhiling Dao 1,* email
1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China 2 Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan, China 3 Pharmacy and Inspection Department, Sichuan Traditional Chinese Medicine College, Mianyang 621000, Sichuan, China 4 The Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China
* Authors to whom correspondence should be addressed.
Received: 31 December 2011; in revised form: 16 January 2012 / Accepted: 17 January 2012 / Published: 31 January 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
PDF Full-text Download PDF Full-Text [126 KB, uploaded 31 January 2012 14:39 CET]
Abstract: Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild individuals from Gaoligong Mountains in western Yunnan Province, China. The number of alleles per locus ranged from 2 to 4 with a mean of 2.9. The expected and observed levels of heterozygosity ranged from 0.042 to 0.726 and from 0.000 to 1.000, with averages of 0.44 and 0.31, respectively. These polymorphic microsatellite markers should prove useful in population genetics studies and assessments of genetic variation to develop conservation and management strategies for this species.
Keywords: Cardiocrinum giganteum var. yunnanense; microsatellite markers; polymorphism; population genetics; Liliaceae

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Li, R.; Yang, J.; Yang, J.; Dao, Z. Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China. Int. J. Mol. Sci. 2012, 13, 1437-1443.

AMA Style

Li R, Yang J, Yang J, Dao Z. Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China. International Journal of Molecular Sciences. 2012; 13(2):1437-1443.

Chicago/Turabian Style

Li, Rong; Yang, Jie; Yang, Junbo; Dao, Zhiling. 2012. "Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China." Int. J. Mol. Sci. 13, no. 2: 1437-1443.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert