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Int. J. Mol. Sci. 2012, 13(2), 1437-1443; doi:10.3390/ijms13021437
Article

Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China

1
,
2,3
,
4,*  and 1,*
Received: 31 December 2011 / Revised: 16 January 2012 / Accepted: 17 January 2012 / Published: 31 January 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild individuals from Gaoligong Mountains in western Yunnan Province, China. The number of alleles per locus ranged from 2 to 4 with a mean of 2.9. The expected and observed levels of heterozygosity ranged from 0.042 to 0.726 and from 0.000 to 1.000, with averages of 0.44 and 0.31, respectively. These polymorphic microsatellite markers should prove useful in population genetics studies and assessments of genetic variation to develop conservation and management strategies for this species.
Keywords: Cardiocrinum giganteum var. yunnanense; microsatellite markers; polymorphism; population genetics; Liliaceae Cardiocrinum giganteum var. yunnanense; microsatellite markers; polymorphism; population genetics; Liliaceae
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Li, R.; Yang, J.; Yang, J.; Dao, Z. Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China. Int. J. Mol. Sci. 2012, 13, 1437-1443.

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