Int. J. Mol. Sci. 2012, 13(2), 1426-1436; doi:10.3390/ijms13021426
Article

Chemical Composition and in Vitro Antifungal Activity Screening of the Allium ursinum L. (Liliaceae)

Received: 28 November 2011; in revised form: 6 January 2012 / Accepted: 19 January 2012 / Published: 30 January 2012
(This article belongs to the Special Issue Biocides)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The objective of the study was to summarize the methods for isolating and identifying natural sulfur compounds from Allium ursinum (ramson) and to discuss the active constituents with regard to antifungal action. Using chromatographic techniques, the active constituents were isolated and subsequently identified. Analyses by high-performance liquid chromatography (HPLC) suggested that these compounds were sulfur constituents, with a characteristic absorbance at 250 nm. Gas chromatography-mass spectrometry (GC-MS) analyses allowed the chemical structures of the isolated constituents to be postulated. We adopted the same methods to identify the health-giving profiling of ramsons and the effects are thought to be primarily derived from the presence and breakdown of the alk(en)ylcysteine sulphoxide, alliin and its subsequent breakdown to allicin (sulfur-compounds of ramson) in connection with antifungal action. The aim of the study was the characterization of the chemical composition of ramsons and the testing of the action of the in vitro extracts, on different strains of Candida albicans. The main goal was to highlight the most efficient extracts of Allium ursinum that can provide long-term antifungal activity without remissions. The extracts from Allium ursinum plants, inhibited growth of Candida spp. cells at concentrations ranging from 0.5 to 4.0 mg/mL, while that of adherent cells at concentrations ranging from 1.0 to > 4.0 mg/mL, depending on the yeast and plant species.
Keywords: ramsons; antifungal activity; extract; HPLC and GC/MS; MBC/MFC
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MDPI and ACS Style

Bagiu, R.V.; Vlaicu, B.; Butnariu, M. Chemical Composition and in Vitro Antifungal Activity Screening of the Allium ursinum L. (Liliaceae). Int. J. Mol. Sci. 2012, 13, 1426-1436.

AMA Style

Bagiu RV, Vlaicu B, Butnariu M. Chemical Composition and in Vitro Antifungal Activity Screening of the Allium ursinum L. (Liliaceae). International Journal of Molecular Sciences. 2012; 13(2):1426-1436.

Chicago/Turabian Style

Bagiu, Radu Vasile; Vlaicu, Brigitha; Butnariu, Monica. 2012. "Chemical Composition and in Vitro Antifungal Activity Screening of the Allium ursinum L. (Liliaceae)." Int. J. Mol. Sci. 13, no. 2: 1426-1436.

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