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Expression Patterns of Glutathione Transferase Gene (GstI) in Maize Seedlings Under Juglone-Induced Oxidative Stress
Department of Biochemistry and Molecular Biology, University of Natural Sciences and Humanities, B. Prusa 12 Street, 08-110 Siedlce, Poland
Received: 9 October 2011; in revised form: 3 November 2011 / Accepted: 8 November 2011 / Published: 16 November 2011
(This article belongs to the Special Issue Biocides
Abstract: Juglone (5-hydroxy-1,4-naphthoquinone) has been identified in organs of many plant species within Juglandaceae family. This secondary metabolite is considered as a highly bioactive substance that functions as direct oxidant stimulating the production of reactive oxygen species (ROS) in acceptor plants. Glutathione transferases (GSTs, E.C.22.214.171.124) represent an important group of cytoprotective enzymes participating in detoxification of xenobiotics and limiting oxidative damages of cellular macromolecules. The purpose of this study was to investigate the impact of tested allelochemical on growth and development of maize (Zea mays L.) seedlings. Furthermore, the effect of juglone-induced oxidative stress on glutathione transferase (GstI) gene expression patterns in maize seedlings was recorded. It was revealed that 4-day juglone treatment significantly stimulated the transcriptional activity of GstI in maize seedlings compared to control plants. By contrast, at the 6th and 8th day of experiments the expression gene responses were slightly lower as compared with non-stressed seedlings. Additionally, the specific gene expression profiles, as well as the inhibition of primary roots and coleoptile elongation were proportional to juglone concentrations. In conclusion, the results provide strong molecular evidence that allelopathic influence of juglone on growth and development of maize seedlings may be relevant with an induction of oxidative stress in acceptor plants.
Keywords: juglone; glutathione transferase gene; real-time quantitative RT-PCR; gene expression; oxidative stress; phytotoxicity; maize
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Sytykiewicz, H. Expression Patterns of Glutathione Transferase Gene (GstI) in Maize Seedlings Under Juglone-Induced Oxidative Stress. Int. J. Mol. Sci. 2011, 12, 7982-7995.
Sytykiewicz H. Expression Patterns of Glutathione Transferase Gene (GstI) in Maize Seedlings Under Juglone-Induced Oxidative Stress. International Journal of Molecular Sciences. 2011; 12(11):7982-7995.
Sytykiewicz, Hubert. 2011. "Expression Patterns of Glutathione Transferase Gene (GstI) in Maize Seedlings Under Juglone-Induced Oxidative Stress." Int. J. Mol. Sci. 12, no. 11: 7982-7995.