- freely available
Int. J. Mol. Sci. 2012, 13(12), 17210-17229; doi:10.3390/ijms131217210
Published: 17 December 2012
Abstract: X-ray Repair Cross Complementing protein 1 (XRCC1) acts as a scaffolding protein in the converging base excision repair (BER) and single strand break repair (SSBR) pathways. XRCC1 also interacts with itself and rapidly accumulates at sites of DNA damage. XRCC1 can thus mediate the assembly of large multiprotein DNA repair complexes as well as facilitate the recruitment of DNA repair proteins to sites of DNA damage. Moreover, XRCC1 is present in constitutive DNA repair complexes, some of which associate with the replication machinery. Because of the critical role of XRCC1 in DNA repair, its common variants Arg194Trp, Arg280His and Arg399Gln have been extensively studied. However, the prevalence of these variants varies strongly in different populations, and their functional influence on DNA repair and disease remains elusive. Here we present the current knowledge about the role of XRCC1 and its variants in BER and human disease/cancer.
1. X-Ray Repair Cross Complementing Protein 1 (XRCC1)
XRCC1 was identified as a single strand break repair (SSBR) factor because transgene expression of human XRCC1 in the Xrcc1 deficient Chinese hamster ovary (CHO) cell line EM9 restored its SSBR capacity . The reduced SSBR capacity of EM9 CHO is associated with a tenfold increase in sensitivity to mono-functional alkylating agents, increased sensitivity to X-ray and ultraviolet (UV) irradiation, and increased sister chromatid exchange (SCE). Although XRCC1-deficient cancer cell lines are viable, XRCC1-deficiency in mice is embryonically lethal . The exact physiological role of XRCC1 during foetal development is difficult to determine because XRCC1-deficient embryos die at around the seventh day, but the arrested embryos resemble those from AP-endonuclase 1 (APE1) deficient mice and die at approximately the same stage as DNA ligase 3 (LIG3) deficient mice embryos [3–5]. However, in addition to BER, XRCC1 has also been linked to non-homologous end joining (NHEJ) and possibly nucleotide excision repair (NER), indicating that the underlying causes for the observed embryonic lethality might be complex [6,7]. While full body Xrcc1 disruption in mice is lethal, Nes-cre conditional Xrcc1 neuron disruption produces viable offspring. However, the Xrcc1Nes–cre mice do not survive longer than 4 months, and while they accumulate DNA strand breaks in neurons none developed cancer within their short lifespan. Xrcc1Nes–cre mice are characterized by delayed growth, smaller brain size, loss of cerebellar interneurons, abnormal hippocampal function, and develop mild ataxia accompanied by episodic seizures . Similar to the XRCC1-deficient CHO cells, cells from XRCC1 deficient mice embryos and Xrcc1Nes–cre mice neurons show hypersensitivity to mutagens [2,8]. Transgene complementation in Xrcc1−/− mice expressing less than 10% of the normal level of XRCC1 is sufficient to rescue the embryonic development and produce healthy fertile adults . However, heterozygous Xrcc1+/− mice, expressing 50% of the normal level of XRCC1, show increased precancerous lesions in the colon and liver toxicity upon ingestion of alkylating agents . This suggests that the cellular concentration of XRCC1 and likely the regulation of associated multiprotein complexes are important for genomic maintenance.
2. Base Excision Repair (BER)
DNA base degradation occurs spontaneously through hydrolysis as well as through interaction with reactive molecules. Endogenous alkylating agents such as S-Adenosyl methionine and exogenous agents such as chemotherapeutic agents and cigarette smoke metabolites add methyl groups to the nitrogens and oxygens of purines and pyrimidines (Figure 1; panel A). Reactive oxygen species (ROS) generated, e.g., endogenously by the electron transport chain in mitochondria, or by ionizing and UV irradiation, typically add hydroxyl groups to double bonded carbons within purines and pyrimidines, as well as the sugar-phosphate DNA backbone causing strand breaks (Figure 1; panel A). Loss of bases and base lesions that alter base pairing properties of DNA are potentially mutagenic and toxic, and merely spontaneous hydrolysis of bases in DNA is estimated to happen more than 18,000 times per cell per day . Despite this high number of DNA damage, the rate of somatic mutations in humans has been estimated to be 10−6 per cell division . The majority of the damaged bases are removed and replaced through BER. Although BER comprises several sub-pathways, it can be generally summarized as five enzymatic steps (Figure 1; panel B). First the N-glycosidic bond of the damaged base is cleaved by a DNA glycosylase leaving an abasic site in the DNA. The sugar-phosphate backbone of the abasic site is then cleaved by a bi-functional glycosylase and/or an AP-endonuclease. If necessary the 3’ strand break end is converted to hydroxyl allowing DNA polymerases to reinsert new bases. Synthesis of a single base is referred to as short patch (SP) BER and synthesis of several bases is referred to as long patch (LP) BER. The 5’ single strand end of the single strand break intermediate is then processed to allow for ligation by DNA ligases. In addition to base damage, the BER pathway is also involved in the repair of abasic sites and single strand breaks that are generated independently of glycosylases and/or AP-endonucleases, such as DNA sugar-phosphate backbone cleavage by ROS or spontaneous hydrolysis of the N-glycosidic bond.
3. XRCC1 Structure and Protein Interacting Regions
The human Xrcc1 gene is 33 kilobases long and located on chromosome 19q13.3–13.3 (Ensembl ID: ENSG00000073050). Its product XRCC1 is a 633 amino acid (aa) long protein of approximately 70 kDa (Uniprot ID: p18887) that is translocated to the nucleus by a nuclear localisation signal (NLS) located between aa 239 and aa 266 (Figure 2; panel B) . XRCC1 is partially excluded from nucleolus and accumulate into discrete foci depending on cell cycle phase and cellular stress [14–16].
The region spanning from aa 1 to aa 183, commonly referred to as N-terminal domain (NTD), binds DNA polymerase beta (POLβ) with high affinity (Figure 2; panels A and B) [17,18]. Nuclear magnetic resonance (NMR) crystallography of the NTD and POLβ has indicated that they form a sandwich like structure that surrounds gapped DNA, suggesting that the NTD might tether XRCC1 to, and protect BER intermediates [19,20]. However, recent NMR studies suggests that POLβ interacts with an oxidized form of NTD in a manner that excludes XRCC1 interaction with gapped DNA . The interaction with XRCC1 stabilizes POLβ by protecting POLβ from degradation, and is required for POLβ recruitment to DNA damage [16,22].
XRCC1 lacks proliferating cell nuclear antigen (PCNA) interaction motifs such as the PCNA interacting peptide box or AlkB homologue 2 PCNA-interacting motif [23,24]. However, the region spanning from aa 166 to aa 310 of purified recombinant XRCC1 has been demonstrated to interact with purified recombinant PCNA (Figure 2; panels A and B) .
The aa 166 to aa 403 region of XRCC1 interacts with nei endonuclease VIII-like 2 (NIEL2), nth endonuclease III-like 1 (NTH1), 8-oxoguanine DNA glycosylase (OGG1), and Uracil-DNA glycosylase 2 (UNG2) [15,25,26]. Furthermore repair activity assays indicate that N-methylpurine DNA glycosylase (MPG) binds within the aa 1 to aa 406 region of XRCC1, although this was not confirmed by immunoblotting . Together, these results indicate that the aa 166 to aa 403 is a central DNA glycosylase interacting region of XRCC1 (Figure 2; panels A and B). In addition, the aa 166 to aa 403 region of XRCC1 appears to interact with APE1 [25,27]. XRCC1 stimulates the activities of recombinant NTH1, OGG1, MPG and APE1 [25–27].
The XRCC1 protein sequence includes two BRCA 1 C terminus (BRCT) domains. One is located centrally, BRCT1 aa 315 to aa 403, and one C-terminally, BRCT2 aa 538 to aa 633 (Figure 2; panel B). BRCT domains are predominantly found among proteins involved in DNA damage response (DDR), and appears to act as phosphorylation dependent protein interaction domains . Of the two XRCC1 BRCT domains, BRCT1 is the most evolutionary conserved and is required for efficient DNA repair and proliferation after DNA methylation damage [29,30]. Merely the NLS and BRCT1 domain (aa 166 to 436) is sufficient to improve the repair proficiency of methylation induced damage of XRCC1 deficient EM9 CHO . XRCC1 BRCT1 interacts with the BRCT domain of Poly(ADP-ribosyl) polymerase 1 (PARP1), with Poly(ADP-ribosyl) polymerase 2 (PARP2), and encompasses a putative Poly(ADP-ribose) binding motif (PARBM: aa 379 to aa 400) (Figure 2; panels A and B) [13,32,33]. The BRCT2 domain of XRCC1 binds the BRCT domain of Ligase 3 (LIG3) [34,35] and, similar to XRCC1 interaction with POLβ, this interaction stabilizes the expression levels of LIG3 (Figure 2; panels A and B) . The XRCC1 BRCT domains also serve as inter-XRCC1 interaction modules, although their respective contribution is still somewhat unclear. Beernink et al. reported in 2005 that XRCC1 BRCT1 domains could form a heterotetrameric interaction with PARP1, while BRCT2 domains could dimerize directly . In 2006, Lévy et al. reported that XRCC1 dimerization was formed through BRCT1 domains and not BRCT2 . In 2011, Cuneo et al. reported a crystallographically resolved structure of a XRCC1/LIG3 tetramer with interaction between the XRCC1 BRCT2 domains . Although the details on how XRCC1 forms multimers are diverging, interaction between XRCC1 deletion mutants containing only BRCT1 as well as both BRCT domains have been confirmed in vivo by fluorescence resonsance energy transfer (FRET) [14,31].
The region between the two XRCC1 BRCT domains (aa 403 to aa 538) interacts with the forkhead-associated (FHA) domains of aprataxin (APTX), polynucleotide kinase/phosphatase (PNKP), and aprataxin and PNKP like factor (APLF) (Figure 2; panels A and B) [40–45]. FHA domains are involved in protein/protein interactions through phospho-threonine binding, and are found in more than 700 eukaryotic proteins such as kinases, phosphatases, kinesins, transcription factors, RNA binding proteins and metabolic enzymes . Interaction with XRCC1 stimulates both the phosphatase and kinase activities of recombinant PNKP .
4. Posttranslational Modifications of XRCC1
The most prominent posttranslational modifications (PTM) of XRCC1, and BER proteins in general, known to date are phosphorylations . XRCC1 is a heavily phosphorylated protein with more than 30 phosphorylated Ser/Thr residues (PhosphoSitePlus database, search term “XRCC1”; p18887. Last accessed 31th October 2012. http://www.phosphosite.org) (Figure 2; panel C). Ser371 within BRCT1 has been shown to be phosphorylated in vivo by DNA-PKcs upon ionizing irradiation induced DNA damage, and to cause XRCC1 dimer dissociation in vitro. However, all other verified phosphorylations of XRCC1 cluster outside of the BRCT domains, mainly within the regions between the NTD and BRCT1 (aa 183 to aa 315), and in the inter BRCT region (aa 403 to aa 538). Checkpoint kinase 2 (CHK2) complexes with XRCC1 and phosphorylates Thr284. XRCC1 mutated at Thr284 was suggested to be linked to accumulation of BER intermediates possibly through modification of XRCC1s interaction with DNA glycosylases . Of the known kinase interactions with XRCC1, casein kinase 2 (CK2) is the most extensively documented. CK2 is a pleiotropic, ubiquitous, and constitutively active kinase. CK2 phosphorylates hundreds of different substrates and among them several factors known to be associated with cellular stress responses, cell proliferation, and cancerogenesis, such as nuclear factor kappa light chain enhancer of activated B cells (NF-kappaB). CK2 expression is up regulated in a wide variety of human cancers and has become an interesting target for drug design . The region between the BRCT domains of XRCC1 encompasses eight primary and five atypical consensus sites for CK2, and they are readily phosphorylated by CK2 in vitro. Nine of the Ser/Thr residues were observed to be phosphorylated in vivo, and among them six residues close to BRCT2 showed reduced phosphorylation when CK2 was knocked down (Figure 2; panel C) [42,43]. Phosphorylations of residues in the inter BRCT region stimulate binding of XRCC1 to the FHA domains of APTX, PNKP, APLF [40–45].
5. XRCC1 Multiprotein Complexes
With the exception of POLβ and LIG3, the remaining XRCC1 binding proteins show extensive overlap in their binding epitopes. Furthermore, only the POLβ and LIG3 interactions are considered strong. This has lead to the notion that XRCC1 contributes to BER as a co-factor or temporary docking platform through a succession of interactions and enzymatic events. However, the dimeric interactions between XRCC1 BRCT domains and heterotetrameric assembly of XRCC1 with either PARP1 or LIG3 observed in vitro as well as by FRET suggest that XRCC1 complexes may be able to contain many factors [14,37–39]. The assembly of large multiprotein complexes is plausible because many of the XRCC1 interacting proteins have been shown to interact among themselves (reviewed in ). In addition, XRCC1 interacts with PCNA, a well-known scaffolding protein (Figure 3).
In 2004 results from our group indicated the presence of large BER proficient multiprotein complexes as UNG2 co-immunoprecipitated with PCNA, XRCC1, and other proteins that bind both XRCC1 and PCNA . Similarly Heale et al., and Parlanti et al. detected XRCC1 in multiprotein complexes associated with Condensin 1 and Cyclin A, in addition to proteins that are not known to interact directly with XRCC1, (e.g., POLδ, POLε, and FEN1) [52,53]. However, co-immunoprecipitation results only suggest the presence of multiprotein complexes as protein interactions may form after cell lysis. The presence of XRCC1 associated multiprotein complexes containing proteins that have overlapping interaction regions within XRCC1 (PCNA and PARP1 vs. UNG2 and APE1) were confirmed by mild in vivo formaldehyde crosslinking prior to co-immunoprecipitation studies. Furthermore, proteins with no known interaction with XRCC1 (POLδ and FEN1) were also found in these complexes . Gel fractionation of cell extracts confirmed the presence of large XRCC1 complexes of various compositions and sizes ranging from 150 to 1500 kDa. The highest BER activity was found in complexes isolated from fractionated extracts ranging in size from 350 to 700 kDa . In vitro studies of interactions between BER enzymes and their DNA substrates indicate that the enzymes stay bound to their product, preventing genotoxic and mutagenic effects of repair intermediates before handing them over to the next enzyme . Multiprotein BER complexes could contribute to tethering of factors to the lesions and repair intermediates and thus provide a platform for coordinated progression of repair, and also increase the rate of repair.
6. The Composition of XRCC1 Associated Multiprotein Complexes
The involvement of replication factors such as POLδ, POLε, FEN1, and PCNA in LP BER, and the deleterious effects of replication past SSBs and certain base lesions have long suggested the presence of replication-coupled BER/SSBR [17,55,56]. The presence of XRCC1 in replication foci and its co-immunoprecipitation with Cyclin A associated proteins further suggest a role for XRCC1 in replication associated repair [14,53]. More recent co-immunoprecipitation data from XRCC1 associated and UNG2 associated complexes isolated from S-phase enriched cells, demonstrated that these complexes contained both common and separate factors. Colocalization studies showed that XRCC1, PNKP and POlβ were found both within and outside of replication foci, while UNG2 was only found in association with the replication machinery. Additionally, the different complexes showed different BER proficiencies. XRCC1-associated complexes were far more efficient in BER than UNG2-associated complexes, indicating the presence of distinct BER complexes during S-phase [15,16].
We have found that the repertoire of proteins recruited to near-UVA (405 nm) induced DNA damage is highly dependent on the radiation dose. Low doses result in recruitment of BER factors that are also observed in foci independent of exogenously induced DNA damage, e.g., PNKP, and POLβ, while high doses in addition resulted in the recruitment of PCNA and FEN1 . In sum, these observations suggest the presence of a “XRCC1 core complex” associated with the replication machinery as well as sites of endogenously and exogenously induced DNA damage (Figure 4).
The strong interaction between XRCC1 and LIG3 and the observed AP-endonuclease activity of low mass XRCC1 associated complexes indicate that the core complex in addition to PNKP and POLβ also contains LIG3 and APE1 [15,16,57,58]. The core complex is extended to include proteins involved in replication, LP BER, and other repair pathways when levels of damage reach a certain threshold. However, while the presence of XRCC1 reduces the near-UVA micro-irradiation doses required for PCNA recruitment, PCNA is recruited to DNA damage in XRCC1 deficient EM9 CHO cells, indicating the involvement of PCNA in DDRs that are independent of XRCC1 [16,31].
PCNA is a central scaffolding protein in the replication machinery as well as in LP BER, and several BER proteins have been demonstrated to co-immunoprecipitate with PCNA (Figure 3) [14,56,59–69]. Interestingly, while the aa 166 to aa 310 region of XRCC1 is required for co-immunoprecipitation of XRCC1 and PCNA, it is not required for colocalization of XRCC1 with PCNA in replication foci during S-phase. The colocalization with PCNA in replication foci requires the aa 310 to aa 436 region, which includes the BRCT1 domain, suggesting that XRCC1 may be recruited to the replication foci via interaction with proteins other than PCNA [14,31]. PARP1, MPG, and UNG2 are all found in replication foci, interact with PCNA, and within or partially with the aa 310 to aa 436 region of XRCC1 [13,15,26,56,66,70].
7. XRCC1 Recruitment to DNA Damage
To date, eleven DNA glycosylases have been identified in humans  (The URL cited in this article is erroneous. An updated table based on this article is available at the following URL, last accessed 18th October 2012. http://sciencepark.mdanderson.org/labs/wood/DNARepairGenes.html#HumanDNARepairGenes). Although the overall folding architectures of DNA glycosylases are similar, their substrate specificities are generally different. How DNA glycosylases are able to detect or get recruited to the plethora of different damaged bases buried within the DNA double helix and chromatin remains elusive, but it involves association to replication, and possibly transcription . We have not been able to induce intracellular re-location of UNG2, MPG or OGG1 to near-UVA micro-irradiated regions, or detect their presence in constitutively present XRCC1 foci, suggesting that these glycosylases are not recruited together with XRCC1. However, this is difficult to assess as most methods for generation of base lesion produce a variety of different lesions that are substrates to different DNA glycosylases, as well as strand breaks that are sensed by PARPs.
Recruitment of XRCC1 to near-UVA micro-irradiated regions of the nucleus is strongly influenced by the aa 166 to aa 310 region and depends on the presence of the BRCT1 domain (aa 315 to aa 403) . While DNA glycosylases interact with both regions, PARP1 and PARP2 interact with XRCC1 within the BRCT1 domain [13,33]. PARP1 and PARP2 bind to and get activated by SSBs [73,74]. Upon activation, PARP1 and PARP2 synthesize branched chains of up to 200 ADP-ribose moieties on themselves and many other target proteins. Poly(ADP)ribose (PAR) chains are rapidly disassembled by PAR glycohydrolase, making PARylation a transient event that last only minutes . The ADP-ribose units are negatively charged and PAR chains thus potentially cause both steric and charge interactions with other molecules . PARylation of histones in response to DNA damage contributes to chromatin reorganization, and PAR chains mediate signalling of the genomic state and serves as recruitment platforms for other proteins . Three PAR association motifs have been described: a macrodomain, a PAR-binding Zinc finger motif, and a cluster of eight amino acids rich in acidic and hydrophobic residues (PARBM). Together these PAR association domains interact with more than 300 proteins, among which DDR proteins are overrepresented in addition to many DNA replication and transcription factors . In addition to the reported PARP1 and PARP2 interaction, a putative PARBM have been identified within the XRCC1 BRCT1 domain, making both PARP1 and PARP2, and PAR chains plausible candidates as initiators of XRCC1 complex recruitment to SSB [13,32]. In 2003 El-Khamisy et al. reported abrogation of XRCC1 foci formation by inhibition of PARylation, and foci reduction by point mutation within the putative PARBM in BRCT1, suggesting that XRCC1 recruitment to SSB is primarily mediated through interaction with PAR chains rather than PARP1 . This was later supported by Mortusewicz et al. and Godon et al. who observed strong reduction of XRCC1 recruitment and increased PARP1 accumulation in near-UVA microirradiation induced foci by inactivation of PARP1s PARylation domain or by use of PARylation inhibitor 4-AN (4-amino-1, 8-naphtalimide; CAS: 1742-95-6 ) [80,81]. However, in our hands treatment with 4-AN or the structurally unrelated PARylation inhibitor PJ34 (CAS: 344458-15-7) caused only minor reduction in XRCC1 recruitment to low dose near-UVA micro-irradiated regions of the nucleus . While XRCC1 accumulation did not coencide with increased accumulation of non-PARylated PARP1, confirming that XRCC1 recruitment is independent of direct interaction with non-PARylated PARP1, they do not support the notion that PARylation activity or PAR chains are required for XRCC1 recruitment to SSBs. The slight reduction of XRCC1 recruitment upon 4-AN and PJ34 treatment might rather be mediated by indirect mechanisms such as PARylation mediated chromatin reorganization.
8. XRCC1 Variants
XRCC1’s role as a scaffolding protein in BER and its observed effect on the activity of certain BER factors have prompted a number of studies assessing the influence of the three most prevalent XRCC1 polymorphisms Arg194Trp (rs1799782), Arg280His (rs25489), and Arg399Gln (rs25487) on genomic stability (Figure 2; panel D) (summary available in ). Studies of primary cells from individuals carrying XRCC1 polymorphisms commonly involve DNA damage induction in heterozygous lymphocytes by alkylating-, oxidizing-, strand break inducing- agents or by ionizing radiation followed by measurement of markers of genome instability such as SCE and micronuclei formation. These studies are limited by sample sizes, and therefore difficult to interpret. Most agents used to induce base lesions also produce lesions or lesion clusters that involve other DNA repair pathways such as NER, trans-lesion synthesis, and double strand break repair (DSBR). Furthermore, dysregulation of any DNA repair mechanism that resolve or intermediately produce strand breaks can be associated with genomic fragmentation and thus influence the studied markers of genomic instability. However, the studies in sum suggest that the Arg194Trp variant is associated with increased genomic stability in response to DNA damaging agents, whereas the Arg280His and Arg399Gln variants are associated with reduced genomic stability (details are available in Table 1 of ). Altered BER efficiency caused by XRCC1 polymorphisms might be mediated by changes in protein-protein interactions, XRCC1 recruitment, or XRCC1 influence on BER enzyme activities. In vivo studies of XCC1 deficient EM9 CHO expressing polymorphic XRCC1 constructs demonstrated that Arg280His and Arg399Gln XRCC1, respectively accumulate less into and dissociates faster from near-UVA micro-irradiation induced DNA damage. Single cell gel electrophoresis indicated that these variants had subtle differences in their repair profiles of oxidative damage induced by hydrogen peroxide, supporting that small changes in the scaffolding properties of XRCC1 may affect DNA repair and thus genomic stability [31,83].
9. XRCC1 and Disease
Base lesions, abasic sites and single strand breaks are relatively common DNA lesions because they are generated continuously by endogenous reactive agents, erroneous DNA processing/synthesis, as well as spontaneously. Minor changes in BER efficiency that alter genomic stability can potentially have a big impact on cancer risk. The Arg194Trp, Arg280His, and Arg399Gln XRCC1 variants have been extensively studied in relation to cancer. However, metastudies of the epidemiological datasets have so far not yielded any unambiguous relationships to cancer prevalence [84–87]. This is probably a result of both the size of populations included in the specific epidemiological studies and significant differences in the genomic single nucleotide polymorphism (SNP) distribution between studied populations (Table 1). For example, homozygous Arg280His has been observed to be associated with breast cancer in Asians, but not in Caucasians . Homozygous Arg194Trp increases risk of lung cancer in Asians, whereas heterozygous Arg194Trp in Caucasians reduces the risk . However, more data regarding XRCC1 and cancer susceptibility is published weekly, and in a recent meta-analysis the Arg399Gln variant was found to be associated with increased risk of developing breast cancer .
DNA synthesis and ligation require 3’ hydroxyl and 5’ phosphate ends. The repair of SSB and BER intermediates typically involve DNA-end processing enzymes, and many have been demonstrated to interact directly or indirectly with XRCC1; e.g., APTX and TDP1, respectively involved in processing 5’ ends produced by abortive ligation, and 3’ ends produced by abortive double stranded unwinding by Topoisomerase 1 [45,91,92]. Mutations in the catalytic domains of TDP1 and APTX are associated with subtypes of the neurodegenerative disease spinocerebellar ataxia [91,93,94]. As previously mentioned, the phosophorylation status of the inter BRCT domain region of XRCC1 (aa 403 to 538) influences the interaction with the FHA domains of PNKP, APTX and APLF. Additionally, PNKP is functionally stimulated by interaction with XRCC1 . XRCC1 thus influences SSB end processing both as an enzymatic co-factor and through its scaffolding properties, and functional changes of XRCC1 could therefore potentially contribute to disease. The observation that conditional neuron disruption of Xrcc1 in mice influences neuronal development and causes accumulation of DNA strand breaks makes it plausible that XRCC1 variants might contribute to neurodegenerative diseases and influence glioma risk . Recent epidemiological studies suggest that Arg399Gln XRCC1 is associated with Parkinson’s disease, sporadic amyotrophic lateral sclerosis, and increased glioma risk [95–98]. Furthermore, consistent with the general observations, the Arg194Trp variant has been found to be associated with a reduced glioma risk . While XRCC1 polymorphisms probably influence BER/SSBR efficiency, it is important to notice that XRCC1 participation in DNA repair is not limited to BER/SSBR. XRCC1 and XRCC1 interacting factors have been shown to be involved in NHEJ and to be recruited to UVC induced DNA damage during NER [6,7,100]. Whether and how different XRCC1 variants affect NHEJ or NER is not known. XRCC1’s contribution to repair of UV induced DNA damage might be affected by the diverging recruitment kinetics of the XRCC1 variants, and this could influence the risk of skin cancer. In accordance with this hypothesis epidemiological studies of XRCC1 variants have associated Arg399Gln and Arg280His with an increased risk of developing melanoma and Arg399Gln and Arg194Trp with an increased risk of developing squamous cell carcinoma [101,102]. However, as noted by Santonocito et al. in 2012, concerning both XRCC1 and OGG1 variants, “all previous papers seemed to exclude possible association between the (non-synonymous) SNPs considered in our study and (malignant melanoma)”. Thus larger mulitcenter studies are required.
In sum, because XRCC1 variants may alter XRCC1’s capacity to form multiprotein complexes, influence the enzymatic activity of protein partners and alter the recruitment to DNA damage, they may influence the risk of disease. However, as XRCC1 is an essential protein during foetal development, a strong negative selection against any prominent functional changes of XRCC1 is likely.
This work is supported by The National Program for Research in Functional Genomics in Norway (FUGE) from The Research Council of Norway, The Cancer Fund at St. Olav’s Hospital, Trondheim, Norway, The Norwegian Cancer Society and The Liaison Committee between the Central Norway Regional Health Authority (RHA) and the Norwegian University of Science and Technology, Norway
- Conflict of InterestThe authors declare no conflict of interest.
- Thompson, L.H.; Brookman, K.W.; Jones, N.J.; Allen, S.A.; Carrano, A.V. Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange. Mol. Cell Biol 1990, 10, 6160–6171. [Google Scholar]
- Tebbs, R.S.; Flannery, M.L.; Meneses, J.J.; Hartmann, A.; Tucker, J.D.; Thompson, L.H.; Cleaver, J.E.; Pedersen, R.A. Requirement for the Xrcc1 DNA base excision repair gene during early mouse development. Dev. Biol 1999, 208, 513–529. [Google Scholar]
- Xanthoudakis, S.; Smeyne, R.J.; Wallace, J.D.; Curran, T. The redox/DNA repair protein, Ref-1, is essential for early embryonic development in mice. Proc. Natl. Acad. Sci. USA 1996, 93, 8919–8923. [Google Scholar]
- Stucki, M.; Pascucci, B.; Parlanti, E.; Fortini, P.; Wilson, S.H.; Hubscher, U.; Dogliotti, E. Mammalian base excision repair by DNA polymerases delta and epsilon. Oncogene 1998, 17, 835–843. [Google Scholar]
- Puebla-Osorio, N.; Lacey, D.B.; Alt, F.W.; Zhu, C. Early embryonic lethality due to targeted inactivation of DNA ligase III. Mol. Cell Biol 2006, 26, 3935–3941. [Google Scholar]
- Audebert, M.; Salles, B.; Calsou, P. Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining. J. Biol. Chem 2004, 279, 55117–55126. [Google Scholar]
- Moser, J.; Kool, H.; Giakzidis, I.; Caldecott, K.; Mullenders, L.H.; Fousteri, M.I. Sealing of chromosomal DNA nicks during nucleotide excision repair requires XRCC1 and DNA ligase III alpha in a cell-cycle-specific manner. Mol. Cell 2007, 27, 311–323. [Google Scholar]
- Lee, Y.; Katyal, S.; Li, Y.; El-Khamisy, S.F.; Russell, H.R.; Caldecott, K.W.; McKinnon, P.J. The genesis of cerebellar interneurons and the prevention of neural DNA damage require XRCC1. Nat. Neurosci 2009, 12, 973–980. [Google Scholar]
- Tebbs, R.S.; Thompson, L.H.; Cleaver, J.E. Rescue of Xrcc1 knockout mouse embryo lethality by transgene-complementation. DNA Repair (Amst.) 2003, 2, 1405–1417. [Google Scholar]
- McNeill, D.R.; Lin, P.C.; Miller, M.G.; Pistell, P.J.; de Souza-Pinto, N.C.; Fishbein, K.W.; Spencer, R.G.; Liu, Y.; Pettan-Brewer, C.; Ladiges, W.C.; et al. XRCC1 haploinsufficiency in mice has little effect on aging, but adversely modifies exposure-dependent susceptibility. Nucleic Acids Res 2011, 39, 7992–8004. [Google Scholar]
- Lindahl, T.; Barnes, D.E. Repair of endogenous DNA damage. Cold Spring Harb. Symp. Quant. Biol 2000, 65, 127–133. [Google Scholar]
- Araten, D.J.; Golde, D.W.; Zhang, R.H.; Thaler, H.T.; Gargiulo, L.; Notaro, R.; Luzzatto, L. A quantitative measurement of the human somatic mutation rate. Cancer Res 2005, 65, 8111–8117. [Google Scholar]
- Masson, M.; Niedergang, C.; Schreiber, V.; Muller, S.; Menissier-de Murcia, J.; de Murcia, G. XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage. Mol. Cell Biol 1998, 18, 3563–3571. [Google Scholar]
- Fan, J.; Otterlei, M.; Wong, H.K.; Tomkinson, A.E.; Wilson, D.M., Jr. XRCC1 co-localizes and physically interacts with PCNA. Nucleic Acids Res. 2004, 32, 2193–2201. [Google Scholar]
- Akbari, M.; Solvang-Garten, K.; Hanssen-Bauer, A.; Lieske, N.V.; Pettersen, H.S.; Pettersen, G.K.; Wilson, D.M., Jr; Krokan, H.E.; Otterlei, M. Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in DNA by XRCC1 complexes. DNA Repair (Amst.) 2010, 9, 785–795. [Google Scholar]
- Hanssen-Bauer, A.; Solvang-Garten, K.; Sundheim, O.; Pena-Diaz, J.; Andersen, S.; Slupphaug, G.; Krokan, H.E.; Wilson, D.M., Jr; Akbari, M.; Otterlei, M. XRCC1 coordinates disparate responses and multiprotein repair complexes depending on the nature and context of the DNA damage. Environ. Mol. Mutagen. 2011, 52, 623–635. [Google Scholar]
- Kubota, Y.; Nash, R.A.; Klungland, A.; Schar, P.; Barnes, D.E.; Lindahl, T. Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein. EMBO J 1996, 15, 6662–6670. [Google Scholar]
- Caldecott, K.W.; Aoufouchi, S.; Johnson, P.; Shall, S. XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular “nick-sensor” in vitro. Nucleic Acids Res 1996, 24, 4387–4394. [Google Scholar]
- Marintchev, A.; Mullen, M.A.; Maciejewski, M.W.; Pan, B.; Gryk, M.R.; Mullen, G.P. Solution structure of the single-strand break repair protein XRCC1 N-terminal domain. Nat. Struct. Biol 1999, 6, 884–893. [Google Scholar]
- Mani, R.S.; Karimi-Busheri, F.; Fanta, M.; Caldecott, K.W.; Cass, C.E.; Weinfeld, M. Biophysical characterization of human XRCC1 and its binding to damaged and undamaged DNA. Biochemistry 2004, 43, 16505–16514. [Google Scholar]
- Cuneo, M.J.; London, R.E. Oxidation state of the XRCC1 N-terminal domain regulates DNA polymerase beta binding affinity. Proc. Natl. Acad. Sci. USA 2010, 107, 6805–6810. [Google Scholar]
- Parsons, J.L.; Tait, P.S.; Finch, D.; Dianova, I.; Allinson, S.L.; Dianov, G.L. CHIP-mediated degradation and DNA damage-dependent stabilization regulate base excision repair proteins. Mol. Cell 2008, 29, 477–487. [Google Scholar]
- Gilljam, K.M.; Feyzi, E.; Aas, P.A.; Sousa, M.M.; Muller, R.; Vagbo, C.B.; Catterall, T.C.; Liabakk, N.B.; Slupphaug, G.; Drablos, F.; et al. Identification of a novel, widespread, and functionally important PCNA-binding motif. J. Cell Biol 2009, 186, 645–654. [Google Scholar]
- Warbrick, E. The puzzle of PCNA’s many partners. Bioessays 2000, 22, 997–1006. [Google Scholar]
- Marsin, S.; Vidal, A.E.; Sossou, M.; Menissier-de Murcia, J.; Le Page, F.; Boiteux, S.; de Murcia, G.; Radicella, J.P. Role of XRCC1 in the coordination and stimulation of oxidative DNA damage repair initiated by the DNA glycosylase hOGG1. J. Biol. Chem 2003, 278, 44068–44074. [Google Scholar]
- Campalans, A.; Marsin, S.; Nakabeppu, Y.; O’Connor, T.R.; Boiteux, S.; Radicella, J.P. XRCC1 interactions with multiple DNA glycosylases: A model for its recruitment to base excision repair. DNA Repair (Amst.) 2005, 4, 826–835. [Google Scholar]
- Vidal, A.E.; Boiteux, S.; Hickson, I.D.; Radicella, J.P. XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions. EMBO J 2001, 20, 6530–6539. [Google Scholar]
- Bork, P.; Hofmann, K.; Bucher, P.; Neuwald, A.F.; Altschul, S.F.; Koonin, E.V. A superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins. FASEB J 1997, 11, 68–76. [Google Scholar]
- Taylor, R.M.; Thistlethwaite, A.; Caldecott, K.W. Central role for the XRCC1 BRCT I domain in mammalian DNA single-strand break repair. Mol. Cell Biol 2002, 22, 2556–2563. [Google Scholar]
- Kubota, Y.; Horiuchi, S. Independent roles of XRCC1’s two BRCT motifs in recovery from methylation damage. DNA Repair (Amst.) 2003, 2, 407–415. [Google Scholar]
- Hanssen-Bauer, A.; Solvang-Garten, K.; Gilljam, K.M.; Torseth, K.; Wilson, D.M., Jr; Akbari, M.; Otterlei, M. The region of XRCC1 which harbours the three most common nonsynonymous polymorphic variants, is essential for the scaffolding function of XRCC1. DNA Repair (Amst.) 2012, 11, 357–366. [Google Scholar]
- Pleschke, J.M.; Kleczkowska, H.E.; Strohm, M.; Althaus, F.R. Poly(ADP-ribose) binds to specific domains in DNA damage checkpoint proteins. J. Biol. Chem 2000, 275, 40974–40980. [Google Scholar]
- Schreiber, V.; Ame, J.C.; Dolle, P.; Schultz, I.; Rinaldi, B.; Fraulob, V.; Menissier-de Murcia, J.; de Murcia, G. Poly(ADP-ribose) polymerase-2 (PARP-2) is required for efficient base excision DNA repair in association with PARP-1 and XRCC1. J. Biol. Chem 2002, 277, 23028–23036. [Google Scholar]
- Nash, R.A.; Caldecott, K.W.; Barnes, D.E.; Lindahl, T. XRCC1 protein interacts with one of two distinct forms of DNA ligase III. Biochemistry 1997, 36, 5207–5211. [Google Scholar]
- Taylor, R.M.; Wickstead, B.; Cronin, S.; Caldecott, K.W. Role of a BRCT domain in the interaction of DNA ligase III-alpha with the DNA repair protein XRCC1. Curr. Biol 1998, 8, 877–880. [Google Scholar]
- Caldecott, K.W.; Tucker, J.D.; Stanker, L.H.; Thompson, L.H. Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells. Nucleic Acids Res 1995, 23, 4836–4843. [Google Scholar]
- Beernink, P.T.; Hwang, M.; Ramirez, M.; Murphy, M.B.; Doyle, S.A.; Thelen, M.P. Specificity of protein interactions mediated by BRCT domains of the XRCC1 DNA repair protein. J. Biol. Chem 2005, 280, 30206–30213. [Google Scholar]
- Levy, N.; Martz, A.; Bresson, A.; Spenlehauer, C.; de Murcia, G.; Menissier-de Murcia, J. XRCC1 is phosphorylated by DNA-dependent protein kinase in response to DNA damage. Nucleic Acids Res 2006, 34, 32–41. [Google Scholar]
- Cuneo, M.J.; Gabel, S.A.; Krahn, J.M.; Ricker, M.A.; London, R.E. The structural basis for partitioning of the XRCC1/DNA ligase III-alpha BRCT-mediated dimer complexes. Nucleic Acids Res. 2011. [Google Scholar] [CrossRef]
- Bekker-Jensen, S.; Fugger, K.; Danielsen, J.R.; Gromova, I.; Sehested, M.; Celis, J.; Bartek, J.; Lukas, J.; Mailand, N. Human Xip1 (C2orf13) is a novel regulator of cellular responses to DNA strand breaks. J. Biol. Chem 2007, 282, 19638–19643. [Google Scholar]
- Iles, N.; Rulten, S.; El-Khamisy, S.F.; Caldecott, K.W. APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks. Mol. Cell Biol 2007, 27, 3793–3803. [Google Scholar]
- Loizou, J.I.; El-Khamisy, S.F.; Zlatanou, A.; Moore, D.J.; Chan, D.W.; Qin, J.; Sarno, S.; Meggio, F.; Pinna, L.A.; Caldecott, K.W. The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks. Cell 2004, 117, 17–28. [Google Scholar]
- Luo, H.; Chan, D.W.; Yang, T.; Rodriguez, M.; Chen, B.P.; Leng, M.; Mu, J.J.; Chen, D.; Songyang, Z.; Wang, Y.; Qin, J. A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment. Mol. Cell Biol 2004, 24, 8356–8365. [Google Scholar]
- Kanno, S.; Kuzuoka, H.; Sasao, S.; Hong, Z.; Lan, L.; Nakajima, S.; Yasui, A. A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. EMBO J 2007, 26, 2094–2103. [Google Scholar]
- Date, H.; Igarashi, S.; Sano, Y.; Takahashi, T.; Takano, H.; Tsuji, S.; Nishizawa, M.; Onodera, O. The FHA domain of aprataxin interacts with the C-terminal region of XRCC1. Biochem. Biophys. Res. Commun 2004, 325, 1279–1285. [Google Scholar]
- Hofmann, K.; Bucher, P. The FHA domain: A putative nuclear signalling domain found in protein kinases and transcription factors. Trends Biochem. Sci 1995, 20, 347–349. [Google Scholar]
- Whitehouse, C.J.; Taylor, R.M.; Thistlethwaite, A.; Zhang, H.; Karimi-Busheri, F.; Lasko, D.D.; Weinfeld, M.; Caldecott, K.W. XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair. Cell 2001, 104, 107–117. [Google Scholar]
- Almeida, K.H.; Sobol, R.W. A unified view of base excision repair: Lesion-dependent protein complexes regulated by post-translational modification. DNA Repair (Amst.) 2007, 6, 695–711. [Google Scholar]
- Chou, W.C.; Wang, H.C.; Wong, F.H.; Ding, S.L.; Wu, P.E.; Shieh, S.Y.; Shen, C.Y. Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair. EMBO J 2008, 27, 3140–3150. [Google Scholar]
- Hanif, I.M.; Shazib, M.A.; Ahmad, K.A.; Pervaiz, S. Casein Kinase II: An attractive target for anti-cancer drug design. Int. J. Biochem. Cell Biol 2010, 42, 1602–1605. [Google Scholar]
- Akbari, M.; Otterlei, M.; Pena-Diaz, J.; Aas, P.A.; Kavli, B.; Liabakk, N.B.; Hagen, L.; Imai, K.; Durandy, A.; Slupphaug, G.; Krokan, H.E. Repair of U/G and U/A in DNA by UNG2-associated repair complexes takes place predominantly by short-patch repair both in proliferating and growth-arrested cells. Nucleic Acids Res 2004, 32, 5486–5498. [Google Scholar]
- Heale, J.T.; Ball, A.R., Jr; Schmiesing, J.A.; Kim, J.S.; Kong, X.; Zhou, S.; Hudson, D.F.; Earnshaw, W.C.; Yokomori, K. Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair. Mol. Cell 2006, 21, 837–848. [Google Scholar]
- Parlanti, E.; Locatelli, G.; Maga, G.; Dogliotti, E. Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins. Nucleic Acids Res 2007, 35, 1569–1577. [Google Scholar]
- Prasad, R.; Shock, D.D.; Beard, W.A.; Wilson, S.H. Substrate channeling in mammalian base excision repair pathways: Passing the baton. J. Biol. Chem 2010, 285, 40479–40488. [Google Scholar]
- Caldecott, K.W. Mammalian DNA single-strand break repair: An X-ra(y)ted affair. Bioessays 2001, 23, 447–455. [Google Scholar]
- Otterlei, M.; Warbrick, E.; Nagelhus, T.A.; Haug, T.; Slupphaug, G.; Akbari, M.; Aas, P.A.; Steinsbekk, K.; Bakke, O.; Krokan, H.E. Post-replicative base excision repair in replication foci. EMBO J 1999, 18, 3834–3844. [Google Scholar]
- Caldecott, K.W.; McKeown, C.K.; Tucker, J.D.; Ljungquist, S.; Thompson, L.H. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III. Mol. Cell Biol 1994, 14, 68–76. [Google Scholar]
- Akbari, M.; Krokan, H.E. Base excision repair efficiency and mechanism in nuclear extracts are influenced by the ratio between volume of nuclear extraction buffer and nuclei-implications for comparative studies. Mutat. Res 2011, 736, 33–38. [Google Scholar]
- Eissenberg, J.C.; Ayyagari, R.; Gomes, X.V.; Burgers, P.M. Mutations in yeast proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon. Mol. Cell Biol 1997, 17, 6367–6378. [Google Scholar]
- Levin, D.S.; Bai, W.; Yao, N.; O’Donnell, M.; Tomkinson, A.E. An interaction between DNA ligase I and proliferating cell nuclear antigen: Implications for Okazaki fragment synthesis and joining. Proc. Natl. Acad. Sci. USA 1997, 94, 12863–12868. [Google Scholar]
- Zhang, P.; Mo, J.Y.; Perez, A.; Leon, A.; Liu, L.; Mazloum, N.; Xu, H.; Lee, M.Y. Direct interaction of proliferating cell nuclear antigen with the p125 catalytic subunit of mammalian DNA polymerase delta. J. Biol. Chem 1999, 274, 26647–26653. [Google Scholar]
- Parker, A.; Gu, Y.; Mahoney, W.; Lee, S.H.; Singh, K.K.; Lu, A.L. Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair. J. Biol. Chem 2001, 276, 5547–5555. [Google Scholar]
- Kedar, P.S.; Kim, S.J.; Robertson, A.; Hou, E.; Prasad, R.; Horton, J.K.; Wilson, S.H. Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen. J. Biol. Chem 2002, 277, 31115–31123. [Google Scholar]
- Frouin, I.; Maga, G.; Denegri, M.; Riva, F.; Savio, M.; Spadari, S.; Prosperi, E.; Scovassi, A.I. Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners. J. Biol. Chem 2003, 278, 39265–39268. [Google Scholar]
- Oyama, M.; Wakasugi, M.; Hama, T.; Hashidume, H.; Iwakami, Y.; Imai, R.; Hoshino, S.; Morioka, H.; Ishigaki, Y.; Nikaido, O.; Matsunaga, T. Human NTH1 physically interacts with p53 and proliferating cell nuclear antigen. Biochem. Biophys. Res. Commun 2004, 321, 183–191. [Google Scholar]
- Xia, L.; Zheng, L.; Lee, H.W.; Bates, S.E.; Federico, L.; Shen, B.; O’Connor, T.R. Human 3-methyladenine-DNA glycosylase: Effect of sequence context on excision, association with PCNA, and stimulation by AP endonuclease. J. Mol. Biol 2005, 346, 1259–1274. [Google Scholar]
- Li, X.; Li, J.; Harrington, J.; Lieber, M.R.; Burgers, P.M. Lagging strand DNA synthesis at the eukaryotic replication fork involves binding and stimulation of FEN-1 by proliferating cell nuclear antigen. J. Biol. Chem 1995, 270, 22109–22112. [Google Scholar]
- Loor, G.; Zhang, S.J.; Zhang, P.; Toomey, N.L.; Lee, M.Y. Identification of DNA replication and cell cycle proteins that interact with PCNA. Nucleic Acids Res 1997, 25, 5041–5046. [Google Scholar]
- Dou, H.; Theriot, C.A.; Das, A.; Hegde, M.L.; Matsumoto, Y.; Boldogh, I.; Hazra, T.K.; Bhakat, K.K.; Mitra, S. Interaction of the human DNA glycosylase NEIL1 with proliferating cell nuclear antigen. The potential for replication-associated repair of oxidized bases in mammalian genomes. J. Biol. Chem 2008, 283, 3130–3140. [Google Scholar]
- Bryant, H.E.; Petermann, E.; Schultz, N.; Jemth, A.S.; Loseva, O.; Issaeva, N.; Johansson, F.; Fernandez, S.; McGlynn, P.; Helleday, T. PARP is activated at stalled forks to mediate Mre11-dependent replication restart and recombination. EMBO J 2009, 28, 2601–2615. [Google Scholar]
- Wood, R.D.; Mitchell, M.; Lindahl, T. Human DNA repair genes, 2005. Mutat. Res 2005, 577, 275–283. [Google Scholar]
- Odell, I.D.; Wallace, S.S.; Pederson, D.S. Rules of engagement for base excision repair in chromatin. J. Cell Physiol 2012, 228, 258–266. [Google Scholar]
- Ame, J.C.; Rolli, V.; Schreiber, V.; Niedergang, C.; Apiou, F.; Decker, P.; Muller, S.; Hoger, T.; Menissier-de Murcia, J.; de Murcia, G. PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase. J. Biol. Chem 1999, 274, 17860–17868. [Google Scholar]
- Gradwohl, G.; Menissier de Murcia, J.M.; Molinete, M.; Simonin, F.; Koken, M.; Hoeijmakers, J.H.; de Murcia, G. The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA. Proc. Natl. Acad. Sci. USA 1990, 87, 2990–2994. [Google Scholar]
- Okano, S.; Lan, L.; Caldecott, K.W.; Mori, T.; Yasui, A. Spatial and temporal cellular responses to single-strand breaks in human cells. Mol. Cell Biol 2003, 23, 3974–3981. [Google Scholar]
- D’Amours, D.; Desnoyers, S.; D’Silva, I.; Poirier, G.G. Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions. Biochem. J. 1999, 342(Pt 2), 249–268. [Google Scholar]
- Schreiber, V.; Dantzer, F.; Ame, J.C.; de Murcia, G. Poly(ADP-ribose): Novel functions for an old molecule. Nat. Rev. Mol. Cell Biol 2006, 7, 517–528. [Google Scholar]
- Gagne, J.P.; Isabelle, M.; Lo, K.S.; Bourassa, S.; Hendzel, M.J.; Dawson, V.L.; Dawson, T.M.; Poirier, G.G. Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. Nucleic Acids Res 2008, 36, 6959–6976. [Google Scholar]
- El-Khamisy, S.F.; Masutani, M.; Suzuki, H.; Caldecott, K.W. A requirement for PARP-1 for the assembly or stability of XRCC1 nuclear foci at sites of oxidative DNA damage. Nucleic Acids Res 2003, 31, 5526–5533. [Google Scholar]
- Mortusewicz, O.; Ame, J.C.; Schreiber, V.; Leonhardt, H. Feedback-regulated poly(ADP-ribosyl)ation by PARP-1 is required for rapid response to DNA damage in living cells. Nucleic Acids Res 2007, 35, 7665–7675. [Google Scholar]
- Godon, C.; Cordelieres, F.P.; Biard, D.; Giocanti, N.; Megnin-Chanet, F.; Hall, J.; Favaudon, V. PARP inhibition versus PARP-1 silencing: Different outcomes in terms of single-strand break repair and radiation susceptibility. Nucleic Acids Res 2008, 36, 4454–4464. [Google Scholar]
- Ginsberg, G.; Angle, K.; Guyton, K.; Sonawane, B. Polymorphism in the DNA repair enzyme XRCC1: Utility of current database and implications for human health risk assessment. Mutat. Res 2011, 727, 1–15. [Google Scholar]
- Berquist, B.R.; Singh, D.K.; Fan, J.; Kim, D.; Gillenwater, E.; Kulkarni, A.; Bohr, V.A.; Ackerman, E.J.; Tomkinson, A.E.; Wilson, D.M. Functional capacity of XRCC1 protein variants identified in DNA repair-deficient Chinese hamster ovary cell lines and the human population. Nucleic Acids Res 2010, 38, 5023–5035. [Google Scholar]
- Xue, H.; Ni, P.; Lin, B.; Xu, H.; Huang, G. X-ray repair cross-complementing group 1 (XRCC1) genetic polymorphisms and gastric cancer risk: A HuGE review and meta-analysis. Am. J. Epidemiol 2011, 173, 363–375. [Google Scholar]
- Huang, J.; Zhang, J.; Zhao, Y.; Liao, B.; Liu, J.; Li, L.; Liao, M.; Wang, L. The Arg194Trp polymorphism in the XRCC1 gene and cancer risk in Chinese Mainland population: A meta-analysis. Mol. Biol. Rep 2011, 38, 4565–4573. [Google Scholar]
- Gsur, A.; Bernhart, K.; Baierl, A.; Feik, E.; Fuhrlinger, G.; Hofer, P.; Leeb, G.; Mach, K.; Micksche, M. No association of XRCC1 polymorphisms Arg194Trp and Arg399Gln with colorectal cancer risk. Cancer Epidemiol 2011, 35, e38–e41. [Google Scholar]
- Chen, B.; Zhou, Y.; Yang, P.; Wu, X.T. Polymorphisms of XRCC1 and gastric cancer susceptibility: A meta-analysis. Mol. Biol. Rep 2011, 39, 1305–1313. [Google Scholar]
- Li, H.; Ha, T.C.; Tai, B.C. XRCC1 gene polymorphisms and breast cancer risk in different populations: A meta-analysis. Breast 2009, 18, 183–191. [Google Scholar]
- Jiang, J.; Liang, X.; Zhou, X.; Huang, R.; Chu, Z.; Zhan, Q.; Lin, H. DNA repair gene X-ray repair cross complementing group 1 Arg194Trp polymorphism on the risk of lung cancer: A meta-analysis on 22 studies. J. Thorac. Oncol 2010, 5, 1741–1747. [Google Scholar]
- Wu, K.; Su, D.; Lin, K.; Luo, J.; Au, W.W. XRCC1 Arg399Gln gene polymorphism and breast cancer risk: A meta-analysis based on case-control studies. Asian Pac. J. Cancer Prev 2011, 12, 2237–2243. [Google Scholar]
- El-Khamisy, S.F.; Saifi, G.M.; Weinfeld, M.; Johansson, F.; Helleday, T.; Lupski, J.R.; Caldecott, K.W. Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1. Nature 2005, 434, 108–113. [Google Scholar]
- Plo, I.; Liao, Z.Y.; Barcelo, J.M.; Kohlhagen, G.; Caldecott, K.W.; Weinfeld, M.; Pommier, Y. Association of XRCC1 and tyrosyl DNA phosphodiesterase (Tdp1) for the repair of topoisomerase I-mediated DNA lesions. DNA Repair (Amst.) 2003, 2, 1087–1100. [Google Scholar]
- Date, H.; Onodera, O.; Tanaka, H.; Iwabuchi, K.; Uekawa, K.; Igarashi, S.; Koike, R.; Hiroi, T.; Yuasa, T.; Awaya, Y.; et al. Early-onset ataxia with ocular motor apraxia and hypoalbuminemia is caused by mutations in a new HIT superfamily gene. Nat. Genet 2001, 29, 184–188. [Google Scholar]
- Moreira, M.C.; Barbot, C.; Tachi, N.; Kozuka, N.; Uchida, E.; Gibson, T.; Mendonca, P.; Costa, M.; Barros, J.; Yanagisawa, T.; et al. The gene mutated in ataxia-ocular apraxia 1 encodes the new HIT/Zn-finger protein aprataxin. Nat. Genet 2001, 29, 189–193. [Google Scholar]
- Wei, X.; Chen, D.; Lv, T. A functional polymorphism in XRCC1 is associated with glioma risk: Evidence from a meta-analysis. Mol. Biol. Rep. 2012. [Google Scholar] [CrossRef]
- Gencer, M.; Dasdemir, S.; Cakmakoglu, B.; Cetinkaya, Y.; Varlibas, F.; Tireli, H.; Kucukali, C.I.; Ozkok, E.; Aydin, M. DNA repair genes in Parkinson’s disease. Genet. Test Mol. Biomarkers 2012, 16, 504–507. [Google Scholar]
- Coppede, F.; Migheli, F.; Lo Gerfo, A.; Fabbrizi, M.R.; Carlesi, C.; Mancuso, M.; Corti, S.; Mezzina, N.; del Bo, R.; Comi, G.P.; et al. Association study between XRCC1 gene polymorphisms and sporadic amyotrophic lateral sclerosis. Amyotroph. Lateral Scler 2010, 11, 122–124. [Google Scholar]
- Yosunkaya, E.; Kucukyuruk, B.; Onaran, I.; Gurel, C.B.; Uzan, M.; Kanigur-Sultuybek, G. Glioma risk associates with polymorphisms of DNA repair genes, XRCC1 and PARP1. Br. J. Neurosurg 2010, 24, 561–565. [Google Scholar]
- Rajaraman, P.; Hutchinson, A.; Wichner, S.; Black, P.M.; Fine, H.A.; Loeffler, J.S.; Selker, R.G.; Shapiro, W.R.; Rothman, N.; Linet, M.S.; Inskip, P.D. DNA repair gene polymorphisms and risk of adult meningioma, glioma, and acoustic neuroma. Neuro-oncology 2010, 12, 37–48. [Google Scholar]
- Audebert, M.; Salles, B.; Weinfeld, M.; Calsou, P. Involvement of polynucleotide kinase in a poly(ADP-ribose) polymerase-1-dependent DNA double-strand breaks rejoining pathway. J. Mol. Biol 2006, 356, 257–265. [Google Scholar]
- Han, J.; Hankinson, S.E.; Colditz, G.A.; Hunter, D.J. Genetic variation in XRCC1, sun exposure, and risk of skin cancer. Br. J. Cancer 2004, 91, 1604–1609. [Google Scholar]
- Santonocito, C.; Scapaticci, M.; Penitente, R.; Paradisi, A.; Capizzi, R.; Lanza-Silveri, S.; Ficarra, S.; Landi, F.; Zuppi, C.; Capoluongo, E. Polymorphisms in base excision DNA repair genes and association with melanoma risk in a pilot study on Central-South Italian population. Clin. Chim. Acta 2012, 413, 1519–1524. [Google Scholar]
|Table 1. Allele frequencies of the three most prevalent Xrcc1 SNPs known to date.|
|aa substitution||SNP ID||Hom.||Het.||Hom.||Het.||Hom.||Het.||Hom.||Het.|
Hom.: homozygous, Het.: heterozygous. Data from the ENSEMBL database. Last accessed 29th November 2012. http://www.ensembl.org.
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).