Int. J. Mol. Sci. 2012, 13(12), 16104-16118; doi:10.3390/ijms131216104
Article

Effects of Curcumin on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Nitric Oxide

email, , , , , email,  and * email
Received: 17 October 2012; in revised form: 19 November 2012 / Accepted: 20 November 2012 / Published: 29 November 2012
(This article belongs to the Section Molecular Diagnostics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Curcumin (diferuloylmethane) is found in the rhizomes of the turmeric plant (Curcuma longa L.) and has been used for centuries as a dietary spice and as a traditional Indian medicine used to treat different conditions. At the cellular level, curcumin modulates important molecular targets: transcription factors, enzymes, cell cycle proteins, cytokines, receptors and cell surface adhesion molecules. Because many of the curcumin targets mentioned above participate in the regulation of bone remodeling, curcumin may affect the skeletal system. Nitric oxide (NO) is a gaseous molecule generated from L-arginine during the catalization of nitric oxide synthase (NOS), and it plays crucial roles in catalization and in the nervous, cardiovascular and immune systems. Human osteoblasts have been shown to express NOS isoforms, and the exact mechanism(s) by which NO regulates bone formation remain unclear. Curcumin has been widely described to inhibit inducible nitric oxide synthase expression and nitric oxide production, at least in part via direct interference in NF-κB activation. In the present study, after exposure of human osteoblast-like cells (MG-63), we have observed that curcumin abrogated inducible NOS expression and decreased NO levels, inhibiting also cell prolifieration. This effect was prevented by the NO donor sodium nitroprusside. Under osteogenic conditions, curcumin also decreased the level of mineralization. Our results indicate that NO plays a role in the osteoblastic profile of MG-63 cells.
Keywords: osteoblast; nitric oxide synthase; nitric oxide
PDF Full-text Download PDF Full-Text [994 KB, uploaded 19 June 2014 04:34 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Moran, J.M.; Roncero-Martin, R.; Rodriguez-Velasco, F.J.; Calderon-Garcia, J.F.; Rey-Sanchez, P.; Vera, V.; Canal-Macias, M.L.; Pedrera-Zamorano, J.D. Effects of Curcumin on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Nitric Oxide. Int. J. Mol. Sci. 2012, 13, 16104-16118.

AMA Style

Moran JM, Roncero-Martin R, Rodriguez-Velasco FJ, Calderon-Garcia JF, Rey-Sanchez P, Vera V, Canal-Macias ML, Pedrera-Zamorano JD. Effects of Curcumin on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Nitric Oxide. International Journal of Molecular Sciences. 2012; 13(12):16104-16118.

Chicago/Turabian Style

Moran, Jose M.; Roncero-Martin, Raul; Rodriguez-Velasco, Francisco J.; Calderon-Garcia, Julian F.; Rey-Sanchez, Purificacion; Vera, Vicente; Canal-Macias, Maria L.; Pedrera-Zamorano, Juan D. 2012. "Effects of Curcumin on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Nitric Oxide." Int. J. Mol. Sci. 13, no. 12: 16104-16118.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert