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Novel Microsatellite Markers of Meretrix petechialis and Cross-species Amplification in Related Taxa (Bivalvia: Veneroida)
Biotechnology Research Division, NFRDI, Busan 619-705, Korea
Southwest Sea Fisheries Research Institute, NFRDI, Yeosu 556-823, Korea
Gyeongsangnam-do Fisheries Resources Research Institute, Tongyoung 650-974, Korea
Department of Parasitology, College of Medicine and UHRC, Inje University, Busan 614-735, Korea
Institute of Environmental Research, Kangwon National University, Chunchon 200-701, Korea
Department of Biological Science, Silla University, Busan 617-736, Korea
* Author to whom correspondence should be addressed.
Received: 17 September 2012; in revised form: 11 November 2012 / Accepted: 14 November 2012 / Published: 28 November 2012
Abstract: The Asian hard clam, Meretrix petechialis, is an economically important bivalve, but its catch and population sizes are decreasing rapidly, owing to many factors, including large-scale reclamation of its natural habitat on the western coast of the Korean peninsula. Attempts to restore the resources and production of this species require genetic structure and diversity information. In this study, we developed 15 microsatellite markers from a partial genomic library enriched in GT repeats. Nine of these markers were polymorphic, with an average allele number of six, and six were monomorphic in 95 tested individuals. No linkage disequilibrium was found between any pair of loci (p > 0.05), and deviations from the Hardy–Weinberg equilibrium (HWE) test showing excess of heterozygotes was observed in only one of nine loci. In addition, no null alleles or genetic differentiation between two tested populations were detected. A cross-species amplification in 12 species of four families resulted in two M. petechialis-specific loci and three possible universal markers. This information will be useful in the future development of high-quality artificial seedlings and sustainable resource management.
Keywords: Meretrix; microsatellite; cross-species; Veneroida
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Kang, J.-H.; Kim, B.-H.; Park, J.-Y.; Lee, J.-M.; Jeong, J.-E.; Lee, J.-S.; Ko, H.-S.; Lee, Y.-S. Novel Microsatellite Markers of Meretrix petechialis and Cross-species Amplification in Related Taxa (Bivalvia: Veneroida). Int. J. Mol. Sci. 2012, 13, 15942-15954.
Kang J-H, Kim B-H, Park J-Y, Lee J-M, Jeong J-E, Lee J-S, Ko H-S, Lee Y-S. Novel Microsatellite Markers of Meretrix petechialis and Cross-species Amplification in Related Taxa (Bivalvia: Veneroida). International Journal of Molecular Sciences. 2012; 13(12):15942-15954.
Kang, Jung-Ha; Kim, Byeng-Hak; Park, Jung-Youn; Lee, Jung-Mi; Jeong, Ji-Eun; Lee, Jun-Sang; Ko, Hyun-Sook; Lee, Yong-Seok. 2012. "Novel Microsatellite Markers of Meretrix petechialis and Cross-species Amplification in Related Taxa (Bivalvia: Veneroida)." Int. J. Mol. Sci. 13, no. 12: 15942-15954.