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Int. J. Mol. Sci. 2012, 13(12), 15653-15667; doi:10.3390/ijms131215653
Article

The Pharmacological NF-κB Inhibitor BAY11-7082 Induces Cell Apoptosis and Inhibits the Migration of Human Uveal Melanoma Cells

1,†
, 1,†
, 2
, 1
, 2
, 2,*  and 1,*
1 Department of Clinical Laboratories, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China 2 Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 24 August 2012 / Revised: 12 November 2012 / Accepted: 19 November 2012 / Published: 23 November 2012
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Uveal melanomas are highly metastatic and have high rate of recurrence due to the lack of effective systemic therapy. The identification of important survival pathways in uveal melanomas provides novel therapeutic targets for effective treatment. In the present study, we found that the NF-κB signaling pathway was constitutively and highly activated in uveal melanoma cells. Treatment with the pharmacological NF-κB specific inhibitor BAY11-7082 markedly decreased the nuclear translocation of NF-κB. In a dose-dependent setting, BAY11-7082 inhibited the proliferation and growth of uveal melanoma cells by inducing apoptosis without effect on cell cycle. The migration capacity of uveal melanoma cells was also significantly suppressed by BAY11-7082 treatment. Mechanistically, BAY11-7082 increased the activity of caspase 3 and reduced the expression of anti-apoptotic protein Bcl-2, but did not influence the expression of pro-apoptotic protein Bax. Furthermore, BAY11-7082 induced uveal melanoma cell apoptosis and inhibited xenograft tumor growth in vivo. Collectively, the present study identified NF-κB as an important survival signal for uveal melanoma cells and suggested that administration of specific NF-κB inhibitor BAY11-7082 could serve as an effective treatment for patients with uveal melanoma.
Keywords: uveal melanoma; NF-κB; BAY11-7082; apoptosis; cell migration uveal melanoma; NF-κB; BAY11-7082; apoptosis; cell migration
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hu, S.; Luo, Q.; Cun, B.; Hu, D.; Ge, S.; Fan, X.; Chen, F. The Pharmacological NF-κB Inhibitor BAY11-7082 Induces Cell Apoptosis and Inhibits the Migration of Human Uveal Melanoma Cells. Int. J. Mol. Sci. 2012, 13, 15653-15667.

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