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Int. J. Mol. Sci. 2012, 13(11), 14929-14945; doi:10.3390/ijms131114929
Review

Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy

 and *
Received: 21 September 2012; in revised form: 31 October 2012 / Accepted: 31 October 2012 / Published: 14 November 2012
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET))
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Abstract: Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly.
Keywords: FRET; FLIM; acceptor photobleaching; RNA interactions; spliceosome FRET; FLIM; acceptor photobleaching; RNA interactions; spliceosome
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Šimková, E.; Staněk, D. Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy. Int. J. Mol. Sci. 2012, 13, 14929-14945.

AMA Style

Šimková E, Staněk D. Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy. International Journal of Molecular Sciences. 2012; 13(11):14929-14945.

Chicago/Turabian Style

Šimková, Eva; Staněk, David. 2012. "Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy." Int. J. Mol. Sci. 13, no. 11: 14929-14945.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert