Int. J. Mol. Sci. 2012, 13(11), 14929-14945; doi:10.3390/ijms131114929
Review

Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy

Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic
* Author to whom correspondence should be addressed.
Received: 21 September 2012; in revised form: 31 October 2012 / Accepted: 31 October 2012 / Published: 14 November 2012
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET))
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Abstract: Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly.
Keywords: FRET; FLIM; acceptor photobleaching; RNA interactions; spliceosome

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MDPI and ACS Style

Šimková, E.; Staněk, D. Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy. Int. J. Mol. Sci. 2012, 13, 14929-14945.

AMA Style

Šimková E, Staněk D. Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy. International Journal of Molecular Sciences. 2012; 13(11):14929-14945.

Chicago/Turabian Style

Šimková, Eva; Staněk, David. 2012. "Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy." Int. J. Mol. Sci. 13, no. 11: 14929-14945.

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