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• Staněk, D
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• Staněk, D
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Int. J. Mol. Sci. 2012, 13(11), 14929-14945; doi:10.3390/ijms131114929

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Probing Nucleic Acid Interactions and Pre-mRNA Splicing by Förster Resonance Energy Transfer (FRET) Microscopy

Eva Šimková and David Staněk*

Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic

* Author to whom correspondence should be addressed.

Received: 21 September 2012; in revised form: 31 October 2012 / Accepted: 31 October 2012 / Published: 14 November 2012

(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET))

Download PDF Full-Text [756 KB, uploaded 14 November 2012 11:58 CET]

Abstract: Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly.

Keywords: FRET; FLIM; acceptor photobleaching; RNA interactions; spliceosome

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