Int. J. Mol. Sci. 2012, 13(11), 14446-14450; doi:10.3390/ijms131114446
Article

Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library

Maha Mezghani-Khemakhem 1,* email, Dhia Bouktila 1,2 email, Nathalie Casse 3 email, Houcine Maaroufi 1 email, Mohamed Makni 1 email and Hanem Makni 1,4 email
1 Genomics of Crop Insect Pests, Faculté des Sciences de Tunis, University of Tunis El-Manar, El Manar 2092, Tunisia 2 Higher Institute of Biotechnology of Béja, University of Jendouba, Avenue Habib Bourguiba, Béja 9000, Tunisia 3 Laboratory Mer, Molécules, Santé (MMS), UFR Sciences and Techniques Le Mans, University of Maine, Le Mans 72085, France 4 Higher Institute of Animation for Youth and Culture, University of Tunis, Bir El Bey 2055, Tunisia
* Author to whom correspondence should be addressed.
Received: 3 September 2012; in revised form: 25 October 2012 / Accepted: 27 October 2012 / Published: 8 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
Keywords: Mayetiola destructor; dinucleotide; trinucleotide; molecular ecology

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MDPI and ACS Style

Mezghani-Khemakhem, M.; Bouktila, D.; Casse, N.; Maaroufi, H.; Makni, M.; Makni, H. Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library. Int. J. Mol. Sci. 2012, 13, 14446-14450.

AMA Style

Mezghani-Khemakhem M, Bouktila D, Casse N, Maaroufi H, Makni M, Makni H. Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library. International Journal of Molecular Sciences. 2012; 13(11):14446-14450.

Chicago/Turabian Style

Mezghani-Khemakhem, Maha; Bouktila, Dhia; Casse, Nathalie; Maaroufi, Houcine; Makni, Mohamed; Makni, Hanem. 2012. "Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library." Int. J. Mol. Sci. 13, no. 11: 14446-14450.

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