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Int. J. Mol. Sci. 2012, 13(11), 14446-14450; doi:10.3390/ijms131114446
Article

Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library

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Received: 3 September 2012 / Revised: 25 October 2012 / Accepted: 27 October 2012 / Published: 8 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
Keywords: Mayetiola destructor; dinucleotide; trinucleotide; molecular ecology Mayetiola destructor; dinucleotide; trinucleotide; molecular ecology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mezghani-Khemakhem, M.; Bouktila, D.; Casse, N.; Maaroufi, H.; Makni, M.; Makni, H. Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library. Int. J. Mol. Sci. 2012, 13, 14446-14450.

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