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Digluconate and Isopropyl Alcohol Biocide Formulation
Microbiology, School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK
Pharmacy and Pharmaceutical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield HD9 3JA, UK
* Author to whom correspondence should be addressed.
Received: 18 September 2012; in revised form: 16 October 2012 / Accepted: 26 October 2012 / Published: 30 October 2012
Abstract: Effective surface disinfection is a fundamental infection control strategy within healthcare. This study assessed the antimicrobial efficacy of novel biocide formulations comprising 5% and 2% eucalyptus oil (EO) combined with 2% chlorhexidine digluconate (CHG) and 70% isopropyl alcohol (IPA) contained within a wipe. The efficacy of this novel antimicrobial formulation to remove and eliminate methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Candida albicans from steel surfaces was investigated. Adpression studies of pre-contaminated wipes were also utilised to assess their potential to induce cross-contamination between hard surfaces. Furthermore, the bactericidal nature of the EO-formulation was established in addition to time-kill. The EO-containing formulations demonstrated bactericidal antimicrobial efficacy against all microorganisms and did not induce surface cross-contamination. There was no significant difference (p < 0.05) between the 5% and 2% EO formulations in their ability to remove microorganisms from steel surfaces, however both significantly (p < 0.05) removed more than the control formulations. Microbial biofilms were eliminated within 10 min (p < 0.05) when exposed to the EO formulations. Our novel EO-formulation demonstrated rapid antimicrobial efficacy for potential disinfection and elimination of microbial biofilms from hard surfaces and may therefore be a useful adjunct to current infection control strategies currently employed within healthcare facilities.
Keywords: eucalyptus oil; green biocide; biofilm; surface disinfection; chlorhexidine digluconate; healthcare associated infection
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Hendry, E.; Conway, B.; Worthington, T. Digluconate and Isopropyl Alcohol Biocide Formulation. Int. J. Mol. Sci. 2012, 13, 14016-14025.
Hendry E, Conway B, Worthington T. Digluconate and Isopropyl Alcohol Biocide Formulation. International Journal of Molecular Sciences. 2012; 13(11):14016-14025.
Hendry, Emma; Conway, Barbara; Worthington, Tony. 2012. "Digluconate and Isopropyl Alcohol Biocide Formulation." Int. J. Mol. Sci. 13, no. 11: 14016-14025.