Int. J. Mol. Sci. 2012, 13(11), 14016-14025; doi:10.3390/ijms131114016

Digluconate and Isopropyl Alcohol Biocide Formulation

1 Microbiology, School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK 2 Pharmacy and Pharmaceutical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield HD9 3JA, UK
* Author to whom correspondence should be addressed.
Received: 18 September 2012; in revised form: 16 October 2012 / Accepted: 26 October 2012 / Published: 30 October 2012
(This article belongs to the Special Issue Green Biocides)
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Abstract: Effective surface disinfection is a fundamental infection control strategy within healthcare. This study assessed the antimicrobial efficacy of novel biocide formulations comprising 5% and 2% eucalyptus oil (EO) combined with 2% chlorhexidine digluconate (CHG) and 70% isopropyl alcohol (IPA) contained within a wipe. The efficacy of this novel antimicrobial formulation to remove and eliminate methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Candida albicans from steel surfaces was investigated. Adpression studies of pre-contaminated wipes were also utilised to assess their potential to induce cross-contamination between hard surfaces. Furthermore, the bactericidal nature of the EO-formulation was established in addition to time-kill. The EO-containing formulations demonstrated bactericidal antimicrobial efficacy against all microorganisms and did not induce surface cross-contamination. There was no significant difference (p < 0.05) between the 5% and 2% EO formulations in their ability to remove microorganisms from steel surfaces, however both significantly (p < 0.05) removed more than the control formulations. Microbial biofilms were eliminated within 10 min (p < 0.05) when exposed to the EO formulations. Our novel EO-formulation demonstrated rapid antimicrobial efficacy for potential disinfection and elimination of microbial biofilms from hard surfaces and may therefore be a useful adjunct to current infection control strategies currently employed within healthcare facilities.
Keywords: eucalyptus oil; green biocide; biofilm; surface disinfection; chlorhexidine digluconate; healthcare associated infection

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MDPI and ACS Style

Hendry, E.; Conway, B.; Worthington, T. Digluconate and Isopropyl Alcohol Biocide Formulation. Int. J. Mol. Sci. 2012, 13, 14016-14025.

AMA Style

Hendry E, Conway B, Worthington T. Digluconate and Isopropyl Alcohol Biocide Formulation. International Journal of Molecular Sciences. 2012; 13(11):14016-14025.

Chicago/Turabian Style

Hendry, Emma; Conway, Barbara; Worthington, Tony. 2012. "Digluconate and Isopropyl Alcohol Biocide Formulation." Int. J. Mol. Sci. 13, no. 11: 14016-14025.

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