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Int. J. Mol. Sci. 2012, 13(11), 13764-13780; doi:10.3390/ijms131113764
Article

Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer

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Received: 13 September 2012; in revised form: 10 October 2012 / Accepted: 16 October 2012 / Published: 24 October 2012
(This article belongs to the Special Issue Advances in Molecular Oncology)
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Abstract: Caveolin-1 (Cav-1) expression deficiency and autophagy in tumor stromal fibroblasts (hereafter fibroblasts) are involved in tumor proliferation and progression, particularly in breast and prostate cancer. The aim of this study was to detect the expression of fibroblastic Cav-1 and LC3B, markers of autophagy, in gastric cancer (GC) and to analyze their clinical significances. Furthermore, because Epstein-Barr virus (EBV)-associated GC (EBVaGC) is a unique subtype of GC; we compared the differential expression of fibroblastic Cav-1 and LC3B in EBVaGC and non-EBVaGC. Quantum dots (QDs)-based immunofluorescence histochemistry was used to examine the expression of fibroblastic Cav-1 and LC3B in 118 cases of GC with adequate stroma. QDs-based double immunofluorescence labeling was performed to detect the coexpression of Cav-1 and LC3B proteins. EBV-encoded small RNA was detected by QDs-based fluorescence in situ hybridization to identify EBVaGC. Multivariate analysis indicated that low fibroblastic Cav-1 level was an independent prognosticator (p = 0.029) that predicted poorer survival of GC patients. Positive fibroblastic LC3B was correlated with lower invasion (p = 0.032) and was positively associated with Cav-1 expression (r = 0.432, p < 0.001). EBV infection did not affect fibroblastic Cav-1 and LC3B expression. In conclusion, positive fibroblastic LC3B correlates with lower invasion, and low expression of fibroblastic Cav-1 is a novel predictor of poor GC prognosis.
Keywords: autophagy; cancer-associated fibroblast; caveolin-1; gastric cancer; light chain 3B; quantum dots; tumor microenvironment autophagy; cancer-associated fibroblast; caveolin-1; gastric cancer; light chain 3B; quantum dots; tumor microenvironment
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

He, Y.; Zhao, X.; Gao, J.; Fan, L.; Yang, G.; Cho, W.C.-S.; Chen, H. Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer. Int. J. Mol. Sci. 2012, 13, 13764-13780.

AMA Style

He Y, Zhao X, Gao J, Fan L, Yang G, Cho WC-S, Chen H. Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer. International Journal of Molecular Sciences. 2012; 13(11):13764-13780.

Chicago/Turabian Style

He, Yuyu; Zhao, Xianda; Gao, Jun; Fan, Lifang; Yang, Guifang; Cho, William C.-S.; Chen, Honglei. 2012. "Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer." Int. J. Mol. Sci. 13, no. 11: 13764-13780.


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