Int. J. Mol. Sci. 2012, 13(10), 13212-13226; doi:10.3390/ijms131013212
Article

Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

1,* email, 2email and 1email
Received: 2 August 2012; in revised form: 19 September 2012 / Accepted: 8 October 2012 / Published: 15 October 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: An heterologous expression of Vitreoscilla hemoglobin (VHb) for improving cell growth and recombinant protein production has been successfully demonstrated in various hosts, including Pichia pastoris. Lower temperature cultures can enhance target protein production in some studies of P. pastoris. In this study, the strategy of combining heterologous VHb expression and lower temperature cultures in P. pastoris showed that final cell density and viability of VHb+ strain at 23 °C were higher than that at 30 °C. In addition, the effects of VHb expression on recombinant β-galactosidase production and oxygen uptake rate were also higher at 23 °C than at 30 °C. Consequently, lower temperature cultures can enlarge VHb effectiveness on cell performance of P. pastoris. This is because VHb activity obtained at 23 °C cultures was twofold higher than that at 30 °C cultures, due to a different heme production. This strategy makes P. pastoris an excellent expression host particularly suitable for increasing the yields of the low-stability and aggregation-prone recombinant proteins.
Keywords: Vitreoscilla hemoglobin; lower temperature; Pichia pastoris; AOX 1 promoter; recombinant protein production
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MDPI and ACS Style

Wu, J.-M.; Wang, S.-Y.; Fu, W.-C. Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris. Int. J. Mol. Sci. 2012, 13, 13212-13226.

AMA Style

Wu J-M, Wang S-Y, Fu W-C. Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris. International Journal of Molecular Sciences. 2012; 13(10):13212-13226.

Chicago/Turabian Style

Wu, Jyh-Ming; Wang, Shin-Yao; Fu, Wei-Chang. 2012. "Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris." Int. J. Mol. Sci. 13, no. 10: 13212-13226.


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