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• Zhang, D
• Tian, A
• Xue, X
• Wang, M
• Qiu, B
• Wu, A
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• Zhang, D
• Tian, A
• Xue, X
• Wang, M
• Qiu, B
• Wu, A
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• Zhang, D
• Tian, A
• Xue, X
• Wang, M
• Qiu, B
• Wu, A

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Int. J. Mol. Sci. 2012, 13(1), 1109-1125; doi:10.3390/ijms13011109

Article

The Effect of Temozolomide/Poly(lactide-co-glycolide) (PLGA)/Nano-Hydroxyapatite Microspheres on Glioma U87 Cells Behavior

Dongyong Zhang ^1,† , Ang Tian ^2,† , Xiangxin Xue ^2,* , Mei Wang ² , Bo Qiu ¹  and Anhua Wu ^1,*

¹ Departments of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 Nanjingbei Street, Heping District, Shenyang 110001, China ² School of Materials and Metallurgy, Northeastern University, No.11, Lane 3, WenHua Road, HePing District, Shenyang 110819, China ^† These authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 7 November 2011; in revised form: 20 December 2011 / Accepted: 11 January 2012 / Published: 19 January 2012

(This article belongs to the Section Material Sciences and Nanotechnology)

Download PDF Full-Text [1891 KB, uploaded 19 January 2012 10:31 CET]

Abstract: In this study, we investigated the effects of temozolomide (TMZ)/Poly (lactide-co-glycolide)(PLGA)/nano-hydroxyapatite microspheres on the behavior of U87 glioma cells. The microspheres were fabricated by the “Solid/Water/Oil” method, and they were characterized by using X-Ray diffraction, scanning electron microscopy and differential scanning calorimetry. The proliferation, apoptosis and invasion of glioma cells were evaluated by MTT, flow cytometry assay and Transwell assay. The presence of the key invasive gene, α_Vβ3 integrin, was detected by the RT-PCR and Western blot method. It was found that the temozolomide/PLGA/nano-hydroxyapatite microspheres have a significantly diminished initial burst of drug release, compared to the TMZ laden PLGA microspheres. Our results suggest they can significantly inhibit the proliferation and invasion of glioma cells, and induce their apoptosis. Additionally, α_Vβ3 integrin was also reduced by the microspheres. These data suggest that by inhibiting the biological behavior of glioma cells in vitro, the newly designed temozolomide/PLGA/nano-hydroxyapatite microspheres, as controlled drug release carriers, have promising potential in treating glioma.

Keywords: controlled release; glioma; hydroxyapatite; Poly (lactide-co-glycolide) PLGA; temozolomide (TMZ)

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