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Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-57, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea
* Author to whom correspondence should be addressed.
Received: 13 September 2011; in revised form: 25 December 2011 / Accepted: 13 January 2012 / Published: 19 January 2012
Abstract: Collective motion of cell sheets plays a role not only in development and repair, but also in devastating diseases such as cancer. However, unlike single-cell motility, collective motion of cell sheets involves complex cell-cell communication during migration; therefore, its mechanism is largely unknown. To elucidate propagation of signaling transduced by cell-cell interaction, we designed a hydrogel substrate that can cause local mechanical stretching of cell sheets. Poly (N-isopropyl acrylamide) (PNIPAAm) hydrogel is a temperature-responsive polymer gel whose volume changes isotropically in response to temperature changes below 37 °C. We designed a combined hydrogel substrate consisting of collagen-immobilized PNIPAAm as the local stimulation side and polyacrylamide (PAAm) as the non-stimulation side to assess propagation of mechanical transduction. Mardin-Darby canine kidney (MDCK) cells adhered to the collagen-immobilized PNIPAAm gel increased it area and were flattened as the gel swelled with temperature decrease. E-cadherin in these cells became undetectable in some domains, and actin stress fibers were more clearly observed at the cell base. In contrast, E-cadherin in cells adhered to the collagen-immobilized PAAm side was equally stained as that in cells adhered to the collagen-immobilized PAAm side even after temperature decrease. ERK1/2 MAPK activation of cells on the non-stimulated substrate occurred after partial stretching of the cell sheet suggesting the propagation of signaling. These results indicate that a change in the balance of mechanical tension induced by partial stretching of cell sheets leads to activation and propagation of the cell signaling.
Keywords: mechanical stimulation; MDCK cell sheets; temperature-responsive hydrogel; collective cell migration; E-cadherin
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Harada, I.; Yanagisawa, S.; Iwasaki, K.; Cho, C.-S.; Akaike, T. Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel. Int. J. Mol. Sci. 2012, 13, 1095-1108.
Harada I, Yanagisawa S, Iwasaki K, Cho C-S, Akaike T. Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel. International Journal of Molecular Sciences. 2012; 13(1):1095-1108.
Harada, Ichiro; Yanagisawa, Shunpei; Iwasaki, Katsuhiko; Cho, Chong-Su; Akaike, Toshihiro. 2012. "Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel." Int. J. Mol. Sci. 13, no. 1: 1095-1108.