Int. J. Mol. Sci. 2012, 13(1), 1095-1108; doi:10.3390/ijms13011095
Article

Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel

1,* email, 1email, 1email, 2email and 1email
Received: 13 September 2011; in revised form: 25 December 2011 / Accepted: 13 January 2012 / Published: 19 January 2012
(This article belongs to the Special Issue Programmable Materials for Mechanobiology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Collective motion of cell sheets plays a role not only in development and repair, but also in devastating diseases such as cancer. However, unlike single-cell motility, collective motion of cell sheets involves complex cell-cell communication during migration; therefore, its mechanism is largely unknown. To elucidate propagation of signaling transduced by cell-cell interaction, we designed a hydrogel substrate that can cause local mechanical stretching of cell sheets. Poly (N-isopropyl acrylamide) (PNIPAAm) hydrogel is a temperature-responsive polymer gel whose volume changes isotropically in response to temperature changes below 37 °C. We designed a combined hydrogel substrate consisting of collagen-immobilized PNIPAAm as the local stimulation side and polyacrylamide (PAAm) as the non-stimulation side to assess propagation of mechanical transduction. Mardin-Darby canine kidney (MDCK) cells adhered to the collagen-immobilized PNIPAAm gel increased it area and were flattened as the gel swelled with temperature decrease. E-cadherin in these cells became undetectable in some domains, and actin stress fibers were more clearly observed at the cell base. In contrast, E-cadherin in cells adhered to the collagen-immobilized PAAm side was equally stained as that in cells adhered to the collagen-immobilized PAAm side even after temperature decrease. ERK1/2 MAPK activation of cells on the non-stimulated substrate occurred after partial stretching of the cell sheet suggesting the propagation of signaling. These results indicate that a change in the balance of mechanical tension induced by partial stretching of cell sheets leads to activation and propagation of the cell signaling.
Keywords: mechanical stimulation; MDCK cell sheets; temperature-responsive hydrogel; collective cell migration; E-cadherin
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MDPI and ACS Style

Harada, I.; Yanagisawa, S.; Iwasaki, K.; Cho, C.-S.; Akaike, T. Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel. Int. J. Mol. Sci. 2012, 13, 1095-1108.

AMA Style

Harada I, Yanagisawa S, Iwasaki K, Cho C-S, Akaike T. Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel. International Journal of Molecular Sciences. 2012; 13(1):1095-1108.

Chicago/Turabian Style

Harada, Ichiro; Yanagisawa, Shunpei; Iwasaki, Katsuhiko; Cho, Chong-Su; Akaike, Toshihiro. 2012. "Local Mechanical Stimulation of Mardin-Darby Canine Kidney Cell Sheets on Temperature-Responsive Hydrogel." Int. J. Mol. Sci. 13, no. 1: 1095-1108.

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