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Int. J. Mol. Sci. 2011, 12(9), 5698-5704; doi:10.3390/ijms12095698
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Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China

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Received: 19 July 2011 / Revised: 18 August 2011 / Accepted: 28 August 2011 / Published: 5 September 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Hibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species.
Keywords: Hibiscus aridicola; endangered plant; SSR markers; population structure; population genetics Hibiscus aridicola; endangered plant; SSR markers; population structure; population genetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhang, L.; Sun, W.; Wang, Z.; Guan, K.; Yang, J. Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China. Int. J. Mol. Sci. 2011, 12, 5698-5704.

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