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Variable Frequencies of Apolipoprotein E Genotypes and Its Effect on Serum Lipids in the Guangxi Zhuang and Han Children
Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei 230022, China
Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530012, China
Department of Nephrology, Peking University Third Hospital, Beijing 100083, China
* Author to whom correspondence should be addressed.
Received: 11 July 2011; in revised form: 15 August 2011 / Accepted: 26 August 2011 / Published: 31 August 2011
Abstract: Guangxi Zhuang, the largest ethnic minority in China, is located in the southern part of the country, and well-known to the world as the longevity village. Studies of apolipoprotein E (APOE) polymorphism in adults suggest the lower frequencies of E4 allele and E4/E4 genotype may account, in part, for the favorable lipid profiles of Guangxi Zhuang. However, the effect of APOE polymorphism on serum lipids in the Guangxi Zhuang children is yet unknown to date. In the present study, genomic DNA was extracted from 278 Guangxi Zhuang and 200 Guangxi Han children. APOE genotypes were determined by PCR-restriction fragment length polymorphism (RFLP) analysis. The fasting serum lipoprotein a [Lp(a)], total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (apoA1) and apoB were measured. Our results demonstrated that no significant differences in serum lipids were observed between the Guangxi Zhuang and Han children. The E4/E4 and E4/E3 genotypic frequencies were significantly lower in the Guangxi Zhuang children compared with the Guangxi Han children, whereas for E2/E2, E3/E2 and E4/E2 genotypic frequencies the opposite was presented. Though no significant differences in serum lipid concentrations were found for variant alleles both in the Guangxi Zhuang and Han children, the trend was observed in the association of higher levels of Lp(a), TC, TG and LDL-C with E4 allele in the Guangxi Zhuang children. In conclusion, a significant heterogeneity in APOE genetic variation indeed exists between the Guangxi Zhuang and Han ethnic group. The E4 allele may serve as a genetic marker for susceptibility to higher lipid profiles in the Guangxi Zhuang children. Lifestyle should be modified, according to APOE polymorphism even in the young children.
Keywords: apolipoprtein; ethnic group; genotype; total cholesterol; child
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Hu, P.; Qin, Y.H.; Lei, F.Y.; Pei, J.; Hu, B.; Lu, L. Variable Frequencies of Apolipoprotein E Genotypes and Its Effect on Serum Lipids in the Guangxi Zhuang and Han Children. Int. J. Mol. Sci. 2011, 12, 5604-5615.
Hu P, Qin YH, Lei FY, Pei J, Hu B, Lu L. Variable Frequencies of Apolipoprotein E Genotypes and Its Effect on Serum Lipids in the Guangxi Zhuang and Han Children. International Journal of Molecular Sciences. 2011; 12(9):5604-5615.
Hu, Peng; Qin, Yuan Han; Lei, Feng Ying; Pei, Juan; Hu, Bo; Lu, Ling. 2011. "Variable Frequencies of Apolipoprotein E Genotypes and Its Effect on Serum Lipids in the Guangxi Zhuang and Han Children." Int. J. Mol. Sci. 12, no. 9: 5604-5615.