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Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species
Department of Biology and Ecology, Faculty of Science and Mathematics, University of Niš, 33, Višegradska, 18000 Niš, Serbia
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, 12, Radoja Domanovića, 34000 Kragujevac, Serbia
* Author to whom correspondence should be addressed.
Received: 23 June 2011; in revised form: 15 August 2011 / Accepted: 19 August 2011 / Published: 23 August 2011
Abstract: The antioxidative, antimicrobial and antiproliferative potentials of the methanol extracts of the lichen species Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri, Hypogymnia physodes and Cladonia foliacea were evaluated. The total phenolic content of the tested extracts varied from 78.12 to 141.59 mg of gallic acid equivalent (GA)/g of extract and the total flavonoid content from 20.14 to 44.43 mg of rutin equivalent (Ru)/g of extract. The antioxidant capacities of the lichen extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging. Hypogymnia physodes with the highest phenolic content showed the strongest DPPH radical scavenging effect. Further, the antimicrobial potential of the lichen extracts was determined by a microdilution method on 29 microorganisms, including 15 strains of bacteria, 10 species of filamentous fungi and 4 yeast species. A high antimicrobial activity of all the tested extracts was observed with more potent inhibitory effects on the growth of Gram (+) bacteria. The highest antimicrobial activity among lichens was demonstrated by Hypogymnia physodes and Cladonia foliacea. Finally, the antiproliferative activity of the lichen extracts was explored on the colon cancer adenocarcinoma cell line HCT-116 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) viability assay and acridine orange/ethidium bromide staining. The methanol extracts of Hypogymnia physodes and Cladonia foliacea showed a better cytotoxic activity than the other extracts. All lichen species showed the ability to induce apoptosis of HCT-116 cells.
Keywords: lichens extract; total phenolic content; antioxidant activity; antimicrobial activity; antiproliferative activity
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Mitrović, T.; Stamenković, S.; Cvetković, V.; Tošić, S.; Stanković, M.; Radojević, I.; Stefanović, O.; Čomić, L.; Đačić, D.; Ćurčić, M.; Marković, S. Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species. Int. J. Mol. Sci. 2011, 12, 5428-5448.
Mitrović T, Stamenković S, Cvetković V, Tošić S, Stanković M, Radojević I, Stefanović O, Čomić L, Đačić D, Ćurčić M, Marković S. Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species. International Journal of Molecular Sciences. 2011; 12(8):5428-5448.
Mitrović, Tatjana; Stamenković, Slaviša; Cvetković, Vladimir; Tošić, Svetlana; Stanković, Milan; Radojević, Ivana; Stefanović, Olgica; Čomić, Ljiljana; Đačić, Dragana; Ćurčić, Milena; Marković, Snežana. 2011. "Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species." Int. J. Mol. Sci. 12, no. 8: 5428-5448.