Next Article in Journal
Epigenetic Therapy for Breast Cancer
Previous Article in Journal
Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2011, 12(7), 4456-4464; doi:10.3390/ijms12074456

Silkworm Hemolymph Down-Regulates the Expression of Endoplasmic Reticulum Chaperones under Radiation-Irradiation

1
Department of Emergency Medicine, Konkuk University Medical Center, Seoul 143-729, Korea
2
Department of Emergency Medicine, Chungnam National University Hospital, Taejon 301-721, Korea
3
Department of Social Spots, Joongbu University, Chungnam 312-702, Korea
4
Department of Anatomy, College of Medicine, Chungnam National University, Taejon 301-747, Korea
5
Division of Life Science, Korea Basic Science Institute, Taejon 305-333, Korea
6
Graduate School of Analytical Science and Technology, Chungnam National University, Taejon 660-758, Korea
7
Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-806, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 25 April 2011 / Revised: 30 May 2011 / Accepted: 24 June 2011 / Published: 8 July 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [466 KB, uploaded 19 June 2014]   |  

Abstract

We demonstrated that up-regulation of gene expression of endoplasmic reticulum (ER) chaperones (BiP, calnexin, calreticulin, ERp29) and ER membrane kinases (IRE1, PERK, ATF6) was induced by radiation in neuronal PC12 cells. However, addition of silkworm, Bombyx mori, hemolymph to irradiated cells resulted in an obvious decrease in expression of these genes, compared with a single radiation treatment. In contrast, one of the ER chaperones, “ischemia-responsive protein 94 kDa” (irp94), was up-regulated by radiation. However, addition of silkworm hemolymph resulted in no change in the expression of irp94, with an expression pattern that differed from that of ER chaperones. Based on these results, we propose that silkworm hemolymph contains factors that regulate a decrease in the expression of ER chaperones under radiation-irradiation conditions, with the exception of irp94, which is not down-regulated. We suggest that this difference in the molecular character of irp94 may provide a clue to the biological functions associated with ER stress pathways, particularly the effects of radiation. View Full-Text
Keywords: radiation; endoplasmic reticulum (ER); chaperone; silkworm hemolymph; ischemia-responsive protein 94 (irp94) radiation; endoplasmic reticulum (ER); chaperone; silkworm hemolymph; ischemia-responsive protein 94 (irp94)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lee, K.R.; Kim, S.-W.; Kim, Y.K.; Kwon, K.; Choi, J.-S.; Yu, K.; Kwon, O.-Y. Silkworm Hemolymph Down-Regulates the Expression of Endoplasmic Reticulum Chaperones under Radiation-Irradiation. Int. J. Mol. Sci. 2011, 12, 4456-4464.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top